Journal Article

Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

ZENG Yinxin YU Yong LI Huirong HE Jianfeng LEE Sang H SUN Kun

2013

Overview

Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their di- versity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization （FISH） showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic （AAP） bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria （Alpha, Beta and Gamma subclasses）, Bac- teroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone li- braries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro- teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.

• Publisher: The Chinese Society of Oceanography,Springer Nature B.V,College of Biological Engineering, Jimei University, Xiamen 361021, China%Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China%Department of Oceanography, Pusan National University, Busan 609735, Korea,Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China
• Subject: Actinobacteria / Bacteria / Bacteroidetes / Biodegradation / Biogeochemistry / Chemical analysis / Climatology / Cloning / Continental margins / Earth and Environmental Science / Earth Sciences / Ecology / Engineering Fluid Dynamics / Environmental Chemistry / Firmicutes / Fluorescence / Fluorescence in situ hybridization / Gammaproteobacteria / Gene sequencing / Hybridization / Libraries / Marine / Marine & Freshwater Sciences / Microbiological strains / Oceanography / Phylogenetics / Phylogeny / Proteobacteria / Proteolysis / Protozoa / Pseudoalteromonas / R&D / Research & development / Ribosomal DNA / rRNA 16S / rRNA基因 / Sea ice / Seawater / Summer / Water analysis / Water sampling / 夏季 / 好氧不产氧光合异养细菌 / 浮游细菌 / 系统发育分析 / 系统发育多样性 / 荧光原位杂交 / 边疆地区