Journal Article

Comparison of ELISA and dual stage real time RT-PCR to differentiate Sabin like and non-Sabin like poliovirus isolates

Sarkate, Purva, Prakash, Charu, Rishi, Narayan, Kaundal, Nirmal

2017

Overview

Environmental surveillance of polioviruses has been used as an important tool in monitoring circulation of wild polioviruses and/or Vaccine derived polioviruses in sewage samples. It is important to distinguish Sabin like isolates from non-Sabin like; ELISA & dual stage real time RT-PCR have been used for the same. Current study was carried out on sewage isolates to compare ELISA & RT-PCR with sequencing to distinguish Sabin like from non-Sabin like. Out of 468 sewage specimens, 91 (19.44%) were non-polio enteroviruses positive and 377 (80.56%) were polio positive by virus isolation method. A total of 488 polio virus isolates were detected by L20B and RD route which were further subjected to ELISA and RT-PCR. The results were compared with sequencing. On comparison, the specificity of ELISA was only 66.67% in spite of very low sensitivity (3.43%). The sensitivity of RT-PCR was 97.71% which makes it a good primary screening test for detection of non-Sabin like viruses. However, the specificity was only 33.33%. RT-PCR appears to be a sensitive tool for detecting non-Sabin like viruses however; the isolates which are non-Sabin like by RT-PCR may not necessarily be mutated viruses. ELISA cannot be used for differentiation of Sabin likes from non-Sabin likes due to low sensitivity.

• Publisher: Springer India,Springer Nature B.V
• Subject: Antibodies / Biochemistry / Biomedical and Life Sciences / Cell Biology / Enterovirus C / environmental monitoring / enzyme-linked immunosorbent assay / Genomes / isolation techniques / Life Sciences / Microbiology / Original / Original Article / Poliomyelitis / Pollutants / Polymerase chain reaction / Protein Structure / quantitative polymerase chain reaction / reverse transcriptase polymerase chain reaction / Sediments / Sewage / Surveillance / Vaccines / Viruses