Journal Article

Retinyl Palmitate Supplementation Modulates T-bet and Interferon Gamma Gene Expression in Multiple Sclerosis Patients

Bitarafan, Sama, Zareei, Mahnaz, Geranmehr, Maziyar, Mohammadzadeh Honarvar, Niyaz, Harirchian, Mohammad Hossein, Saboor-Yaraghi, Ali Akbar, Abdolahi, Mina, Koohdani, Fariba, Siassi, Feridoun, Salehi, Eisa, Sahraian, Mohammad Ali, Chahardoli, Reza, Abedi, Elahe

2016

Overview

Vitamin A derivatives such as retinoic acid may improve the impaired balance of CD4+ T cells in autoimmune and inflammatory diseases. This study is a double-blind randomized trial to evaluate the effect of vitamin A (as form of retinyl palmitate) supplementation on multiple sclerosis (MS) patients. Thirty-nine patients were enrolled and randomly assigned to two groups. Both groups were followed for 6 months. The experimental group received 25,000 IU of retinyl palmitate daily, while the control group received a placebo. Before and after the study, the expression of interferon gamma (IFN-γ) and T-bet genes was evaluated in peripheral blood mononuclear cells of patients by RT-PCR. The results showed that after 6 months of supplementation, expression of IFN-γ and T-bet was significantly decreased. These data suggest that retinyl palmitate supplementation can modulate the impaired balance of Th1 and Th2 cells and vitamin A products that may be involved in the therapeutic mechanism of vitamin A in MS patients. This study provides information regarding the decreased gene expression of IFN-γ and T-bet in MS by retinyl palmitate supplementation.

• Publisher: Springer US,Springer Nature B.V
• Subject: Adult / Biomedical and Life Sciences / Biomedicine / CD4 antigen / Cell Biology / Dogs / Female / Gene expression / Helper cells / Humans / Inflammatory diseases / Interferon / Interferon-gamma - blood / Leukocytes (mononuclear) / Lymphocytes / Lymphocytes T / Male / Multiple sclerosis / Multiple Sclerosis, Relapsing-Remitting - blood / Multiple Sclerosis, Relapsing-Remitting - drug therapy / Neurochemistry / Neurology / Neurosciences / Palmitic acid / Patients / Peripheral blood mononuclear cells / Polymerase chain reaction / Proteomics / Retinoic acid / Supplements / T-Box Domain Proteins - blood / Th1 Cells - drug effects / Th1 Cells - metabolism / Th2 Cells - drug effects / Th2 Cells - metabolism / Vitamin A / Vitamin A - administration & dosage / Vitamin A - adverse effects / Vitamin A - analogs & derivatives / Vitamin A - pharmacology / Vitamin A - therapeutic use / Vitamins - administration & dosage / Vitamins - adverse effects / Vitamins - pharmacology / Vitamins - therapeutic use / γ-Interferon