Journal Article

Salinicoccus halitifaciens sp. nov., a novel bacterium participating in halite formation

Ramana, Chintalapati Venkata, Mukherjee, Tapas, Sasikala, Chintalapati, Kiran, Pemmaraju Usha, Srinivas, Are, Tushar, Lodha, Subhash, Yadav

2013

Overview

Strain JC90 T was isolated from a soda lake in Lonar, India. Strain JC90 T maintains its external pH to 8.5 and participates in halite formation. Based on 16S rRNA gene sequence similarity studies, strain JC90 T was found to belong to the genus Salinicoccus and is most closely related to “ Salinicoccus kekensis ” K164 T (99.3 %), Salinicoccus alkaliphilus T8 T (98.4 %) and other members of the genus Salinicoccus (<96.5 %). However Strain JC90 T is <36 % related (based on DNA–DNA hybridization) with the type strains of “ S . kekensis ” K164 T and S. alkaliphilus T8 T . The DNA G+C content of strain JC90 T was determined to be 46 mol %. The cell-wall amino acids were identified as lysine and glycine. Polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl ethanolamine, an unidentified glycolipid and unidentified lipids (L1,2). Major hopanoids of strain JC90 T were determined to be bacterial hopane derivatives (BHD1,2), diplopterol, diploptene and two unidentified hopanoids (UH1,2). The predominant isoprenoid quinone was identified as menaquinone (MK-6). Anteiso -C 15:0 was determined to be the predominant fatty acid and significant proportions of iso -C 14:0 , C 14:0 , iso -C 15:0 , C 16:0 , iso -C 16:0 , iso -C 17:0 , anteiso -C 17:0 and C 18:0 2OH were also detected. The results of physiological and biochemical tests support the molecular evidence and allowed a clear phenotypic differentiation of strain JC90 T from all other members of the genus Salinicoccus . Strain JC90 T is therefore considered to represent a novel species, for which the name Salinicoccus halitifaciens sp. nov. is proposed. The type strain is JC90 T (=KCTC 13894 T =DSM 25286 T ).

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Amino acids / Amino Acids - analysis / analysis / Bacteria / Bacteriology / Base Composition / Biochemical tests / Biochemistry / Biomedical and Life Sciences / Cell Wall / Cell Wall - chemistry / chemistry / classification / Cluster Analysis / Cytosol / Cytosol - chemistry / Deoxyribonucleic acid / DNA / DNA, Bacterial / DNA, Bacterial - chemistry / DNA, Bacterial - genetics / DNA, Ribosomal / DNA, Ribosomal - chemistry / DNA, Ribosomal - genetics / ethanolamine / fatty acids / Fatty Acids - analysis / genetics / Genotype & phenotype / Hydrocarbons, Halogenated / Hydrocarbons, Halogenated - metabolism / India / isolation & purification / isoprenoids / lakes / Lakes - microbiology / Life Sciences / Lipids / Medical Microbiology / metabolism / Microbiology / Molecular Sequence Data / nucleic acid hybridization / nucleotide sequences / Original Paper / Phospholipids / Phospholipids - analysis / Phylogenetics / Phylogeny / Plant Sciences / Quinones / Quinones - analysis / ribosomal RNA / RNA, Ribosomal, 16S / RNA, Ribosomal, 16S - genetics / Salinicoccus / Sequence Analysis, DNA / Soil Science & Conservation / Staphylococcaceae / Staphylococcaceae - classification / Staphylococcaceae - isolation & purification / Staphylococcaceae - metabolism / Taxonomy / Triterpenes