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Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LR1 isolated from the gastrointestinal tract of fish
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Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LR1 isolated from the gastrointestinal tract of fish
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Journal Article
Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LR1 isolated from the gastrointestinal tract of fish
2017
Overview
In the present investigation, probiotic potential (antagonistic activity, enzyme production, hemolytic activity, biosafety, antibiotic sensitivity and bile tolerance level) of
Bacillus subtilis
LR1 was evaluated. Bacteriocin produced by the bacterial strain
B. subtilis
LR1 isolated from the gastrointestinal tract of
Labeo rohita
was purified and characterized. The molecular weight of the purified bacteriocin was ~50 kDa in 12 % Native PAGE and showed inhibitory activity against four fish pathogens such as
Bacillus mycoides
,
Aeromonas salmonicida
,
Pseudomonas fluorescens
and
Aeromonas hydrophila
. The purified bacteriocin was maximally active at temperature 40 °C and pH 7.0, while none of the tested surfactants affect the bacteriocin activity. Extracellular enzyme activity of the selected bacterial strain was also evaluated. Amylase activity was estimated to be highest (38.23 ± 1.15 µg of maltose liberated mg
−1
protein ml
−1
of culture filtrate) followed by cellulase and protease activity. The selected bacterium was sensitive to most of the antibiotics used in this experiment, can tolerate 0.25 % bile salt and non-hemolytic in nature. Finally, the efficiency of the proposed probiotic candidate was evaluated in in vivo condition. It was detected that the bacterial strain can effectively reduce bacterial pathogenicity in Indian major carps.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ amylases
/ Animals
/ Anti-Bacterial Agents - chemistry
/ Anti-Bacterial Agents - metabolism
/ Anti-Bacterial Agents - pharmacology
/ Bacillus subtilis - classification
/ Bacillus subtilis - enzymology
/ Bacillus subtilis - genetics
/ Bacillus subtilis - isolation & purification
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ carp
/ Disease
/ Ecology
/ Enzymes
/ Filtrate
/ Gastrointestinal Tract - microbiology
/ maltose
/ polyacrylamide gel electrophoresis
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