Journal Article

Protodioscin ameliorates oxidative stress, inflammation and histology outcome in Complete Freund’s adjuvant induced arthritis rats

Hou, Ya-Ling, Liu, Jia-Yu, Cheng, Guo-Hua, Wang, Li, Tu, Ran, Cao, Rong, Zhang, Jun-Li, Qiu, Hong Xia, Liu, Dan

2017

Overview

Protective effect of protodioscin or methyl protodioscin against inflammation had been reported in various inflammation diseases. This study aimed to investigate the effect of protodioscin against Complete Freund’s adjuvant (CFA)-induced arthritis rats. Rats randomly divided into model groups were injected with CFA, companied with different dose of protodioscin (50, 100, and 200 mg/kg body weight). The histology, changes in biochemical parameters and inflammatory cytokines expression were detected for anti-inflammation effect evaluation of protodioscin. CFA treatment induced arthritic rats with swelling paw, ankle inflammation, and area of lymphocyte infiltration, upregulated inflammatory cytokines (IL-1β, TNF-α, cyclo-oxygenase 2, and IL-6 as well as prostaglandin E2), articular elastase, myeloperoxidase, lipid peroxidase and nitrite oxide levels, downregulated glutathione, catalase, and superoxide dismutase. In contrast, protodioscin ameliorated all the changes induced by CFA in rats, suggesting the anti-inflammatory effect of protodioscin. We concluded that protodioscin administration into CFA-induced arthritis rats protected against CFA-induced oxidative stress, neutrophil infiltration, and inflammation, suggesting the anti-inflammatory effect and the therapeutic potential of protodioscin for arthritis.

• Publisher: Springer US,Springer,Springer Nature B.V
• Subject: Animals / Ankle / Anti-Inflammatory Agents - pharmacology / Antiarthritic agents / Apoptosis - drug effects / Arthritis / Arthritis, Experimental - chemically induced / Arthritis, Experimental - drug therapy / Arthritis, Experimental - genetics / Arthritis, Experimental - pathology / Biochemistry / Biomedical and Life Sciences / Biomedicine / Body weight / Cancer Research / Catalase / Catalase - genetics / Catalase - metabolism / Cell Biology / Cyclooxygenase 2 - genetics / Cyclooxygenase 2 - metabolism / Cyclooxygenase-2 / Cytokines / Dinoprostone - metabolism / Diosgenin - analogs & derivatives / Diosgenin - pharmacology / Dose-Response Relationship, Drug / Edema - chemically induced / Edema - drug therapy / Edema - genetics / Edema - pathology / Elastase / Freund's Adjuvant - administration & dosage / Gene Expression Regulation - drug effects / Glutathione / Glutathione - metabolism / Hindlimb / Histology / Infiltration / Inflammation / Interleukin 6 / Interleukin-1beta - genetics / Interleukin-1beta - metabolism / Interleukin-6 - genetics / Interleukin-6 - metabolism / Lipids / Lymphocytes / Male / Nitric Oxide - metabolism / Oncology / Original Paper / Oxidative Stress / Oxygenase / Pancreatic Elastase - genetics / Pancreatic Elastase - metabolism / Peroxidase / Peroxidase - genetics / Peroxidase - metabolism / Prostaglandin E2 / Prostaglandin endoperoxide synthase / Rats / Rats, Sprague-Dawley / Rodents / Saponins - pharmacology / Signal Transduction / Superoxide / Superoxide dismutase / Superoxide Dismutase - genetics / Superoxide Dismutase - metabolism / Tumor Necrosis Factor-alpha - genetics / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-α / Virology