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Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
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Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
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Journal Article
Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
2019
Overview
Macrophages play critical roles in immunity, development, tissue repair, and cancer, but studies of their function have been hampered by poorly-differentiated tumor cell lines and genetically-intractable primary cells. Here we report a facile system for genome editing in non-transformed macrophages by differentiating ER-Hoxb8 myeloid progenitors from Cas9-expressing transgenic mice. These conditionally immortalized macrophages (CIMs) retain characteristics of primary macrophages derived from the bone marrow yet allow for easy genetic manipulation and a virtually unlimited supply of cells. We demonstrate the utility of this system for dissection of host genetics during intracellular bacterial infection using two important human pathogens: Listeria monocytogenes and Mycobacterium tuberculosis.
Publisher
eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
Subject
/ Bone Marrow Cells - immunology
/ Bone Marrow Cells - metabolism
/ Bone Marrow Cells - microbiology
/ CRISPR
/ Genomes
/ Homeodomain Proteins - genetics
/ Homeodomain Proteins - immunology
/ Homeodomain Proteins - metabolism
/ Host-Pathogen Interactions - immunology
/ Humans
/ Ligands
/ Listeria monocytogenes - immunology
/ Listeria monocytogenes - physiology
/ Microbiology and Infectious Disease
/ Mycobacterium tuberculosis - immunology
/ Mycobacterium tuberculosis - physiology
/ Proteins
/ Software
