Journal Article

Cornus officinalis Ethanolic Extract with Potential Anti-Allergic, Anti-Inflammatory, and Antioxidant Activities

Ali, Md. Sekendar, Abbas, Muhammad Aleem, Boby, Naila, Im, Zi-Eum, Quah, Yixian, Lee, Seung-Jin, Lee, Eon-Bee, Birhanu, Biruk Tesfaye, Park, Seung-Chun

2020

Overview

Atopic dermatitis (AD) is an allergic and chronic inflammatory skin disease. The present study investigates the anti-allergic, antioxidant, and anti-inflammatory activities of the ethanolic extract of Cornus officinalis (COFE) for possible applications in the treatment of AD. COFE inhibits the release of β-hexosaminidase from RBL-2H3 cells sensitized with the dinitrophenyl-immunoglobulin E (IgE-DNP) antibody after stimulation with dinitrophenyl-human serum albumin (DNP-HSA) in a concentration-dependent manner (IC50 = 0.178 mg/mL). Antioxidant activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power assay, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, result in EC50 values of 1.82, 10.76, and 0.6 mg/mL, respectively. Moreover, the extract significantly inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production and the mRNA expression of iNOS and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) through attenuation of NF-κB activation in RAW 264.7 cells. COFE significantly inhibits TNF-α-induced apoptosis in HaCaT cells without cytotoxic effects (p < 0.05). Furthermore, 2-furancarboxaldehyde and loganin are identified by gas chromatography/mass spectrometry (GC-MS) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis, respectively, as the major compounds. Molecular docking analysis shows that loganin, cornuside, and naringenin 7-O-β-D-glucoside could potentially disrupt the binding of IgE to human high-affinity IgE receptors (FceRI). Our results suggest that COFE might possess potential inhibitory effects on allergic responses, oxidative stress, and inflammatory responses.

• Publisher: MDPI AG,MDPI
• Subject: 2,2-diphenyl-1-picrylhydrazyl / Animals / anti-allergic agents / Anti-Allergic Agents - pharmacology / Anti-Inflammatory Agents - pharmacology / antibodies / antioxidant activity / Antioxidants / Antioxidants - pharmacology / apoptosis / assays / atopic dermatitis / Chromatography / Chromatography, Liquid / Cornus / Cornus officinalis / Cytokines - metabolism / Cytotoxicity / Dermatitis / Eczema / Ethanol - pharmacology / Free radicals / gas chromatography / gene expression / Humans / interleukin-6 / lipopolysaccharides / Lipopolysaccharides - metabolism / liquid chromatography / loganin / Mass spectrometry / Mice / Molecular Docking Simulation / naringenin / nitric oxide / Nitric Oxide - biosynthesis / oxidative stress / Oxidative Stress - drug effects / Plant Extracts - pharmacology / Proteins / RAW 264.7 Cells / Scientific imaging / serum albumin / Tandem Mass Spectrometry