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Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets

by Skarda, Jozef , Novak, Zdenek , Sinkora, Marek , Tlaskalova-Hogenova, Helena , Stepankova, Renata , Fajstova, Alena , Srutkova, Dagmar , Raskova Kafkova, Leona , Krupka, Michal , Uberall, Ivo , Stepanova, Katerina , Stehlikova, Zuzana , Hermanova, Petra , Brokesova, Diana , Coufal, Stepan , Dvorak, Jiri , Jiraskova Zakostelska, Zuzana , Raska, Milan , Vannucci, Luca , Mestecky, Jiri

in Agglutination / Allergology / Animal models / Animals / Animals, Newborn / Antibodies / Biomedical and Life Sciences / Biomedicine / Deglycosylation / Disease Resistance / E coli / Epithelial cells / Escherichia coli - physiology / Escherichia coli Infections - immunology / Fab / Female / Gastroenterology / Germ-Free Life / Germfree / Glycosylation / Immunoglobulin A / Immunoglobulin A, Secretory - metabolism / Immunoglobulin Fab Fragments - metabolism / Immunology / Intestine / Microbiota / Mucosa / N-glycans / Polysaccharides / Polysaccharides - metabolism / Pregnancy / Secretory component / Single-Chain Antibodies - metabolism / Swine

2021

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2021

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2021

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Journal Article

Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets

Skarda, Jozef,

Novak, Zdenek,

Sinkora, Marek,

Tlaskalova-Hogenova, Helena,

Stepankova, Renata,

Fajstova, Alena,

Srutkova, Dagmar,

Raskova Kafkova, Leona,

Krupka, Michal,

Uberall, Ivo,

Stepanova, Katerina,

Stehlikova, Zuzana,

Hermanova, Petra,

Brokesova, Diana,

Coufal, Stepan,

Dvorak, Jiri,

Jiraskova Zakostelska, Zuzana,

Raska, Milan,

Vannucci, Luca,

Mestecky, Jiri

2021

Overview

Mucosal surfaces are colonized by highly diverse commensal microbiota. Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans. In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo. Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge. SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival. In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55. These findings highlight the role of SIgA-associated N-glycans in protection. Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.

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Elsevier Inc,Nature Publishing Group US,Elsevier Limited

Subject

/ Animals

/ Biomedical and Life Sciences