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LINE-1 transcription activates long-range gene expression
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LINE-1 transcription activates long-range gene expression
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Journal Article
LINE-1 transcription activates long-range gene expression
2024
Overview
Long interspersed nuclear element-1 (LINE-1 or L1) is a retrotransposon group that constitutes 17% of the human genome and shows variable expression across cell types. However, the control of L1 expression and its function in gene regulation are incompletely understood. Here we show that L1 transcription activates long-range gene expression. Genome-wide CRISPR–Cas9 screening using a reporter driven by the L1 5′ UTR in human cells identifies functionally diverse genes affecting L1 expression. Unexpectedly, altering L1 expression by knockout of regulatory genes impacts distant gene expression. L1s can physically contact their distal target genes, with these interactions becoming stronger upon L1 activation and weaker when L1 is silenced. Remarkably, L1s contact and activate genes essential for zygotic genome activation (ZGA), and L1 knockdown impairs ZGA, leading to developmental arrest in mouse embryos. These results characterize the regulation and function of L1 in long-range gene activation and reveal its importance in mammalian ZGA.
A CRISPR–Cas9 screen identifies genes that modulate long interspersed nuclear element-1 (LINE-1) expression in human cells. LINE-1 5′ UTRs have enhancer features and can activate long-range gene expression, including during zygotic genome activation.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 13/100
/ 13/109
/ 14/63
/ 38/22
/ 38/23
/ 38/39
/ 38/47
/ 38/77
/ 38/91
/ 64/60
/ 82/80
/ Animal Genetics and Genomics
/ Animals
/ Biomedical and Life Sciences
/ CRISPR
/ Embryos
/ Genes
/ Genomes
/ Humans
/ Long interspersed nucleotide elements
/ Long Interspersed Nucleotide Elements - genetics
/ Mice
/ Proteins
