Asset Details
Do you wish to reserve the book?
Structure- and mechanism-guided design of single fluorescent protein-based biosensors
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Structure- and mechanism-guided design of single fluorescent protein-based biosensors
Do you wish to request the book?
Structure- and mechanism-guided design of single fluorescent protein-based biosensors
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Structure- and mechanism-guided design of single fluorescent protein-based biosensors
2021
Overview
Intensiometric genetically encoded biosensors, based on allosteric modulation of the fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of neural activities and other cellular biochemical events. The archetypical example of such biosensors is the GCaMP series of Ca
2+
biosensors, which have been steadily improved over the past two decades and are now indispensable tools for neuroscience. However, no other biosensors have reached levels of performance, or had revolutionary impacts within specific disciplines, comparable to that of the Ca
2+
biosensors. Of the many reasons why this has been the case, a critical one has been a general black-box view of biosensor structure and mechanism. With this Perspective, we aim to summarize what is known about biosensor structure and mechanisms and, based on this foundation, provide guidelines to accelerate the development of a broader range of biosensors with performance comparable to that of the GCaMP series.
Using extensive knowledge about the structures and mechanisms of genetically encoded fluorescent biosensors, this Perspective provides guidelines to aid and accelerate the development of an increasingly broad range of high-performance imaging tools.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biosensing Techniques - instrumentation
/ Biosensing Techniques - methods
/ Calcium
/ Chemistry and Materials Science
/ Fluorescence Resonance Energy Transfer - methods
/ Green Fluorescent Proteins - chemistry
/ Green Fluorescent Proteins - genetics
/ Green Fluorescent Proteins - metabolism
/ Humans
/ Oligopeptides - chemical synthesis
/ Proteins
/ Recombinant Fusion Proteins - chemistry
/ Recombinant Fusion Proteins - genetics
