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Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing
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Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing
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Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing
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Journal Article
Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing
2013
Overview
Mammalian testis development is a complex and highly sophisticated process. To study the dynamic change of normal testis development at the transcriptional level, we investigated mouse testes at three postnatal ages： 6 days postnatal, 4 weeks old, and 10 weeks old, representing infant （PN1）, juvenile （PN2）, and adult （PN3） stages, respectively. Using ultra high-throughput RNA sequencing （RNA-seq） technology, we obtained 211 million reads with a length of 35 bp. We identified 18837 genes that were expressed in mouse testes, and found that genes expressed at the highest level were involved in spermatogenesis. The gene expression pattern in PN1 was distinct from that in PN2 and PN3, which indicates that spermatogenesis has commenced in PN2. We analyzed a large number of genes related to spermatogenesis and somatic development of the testis, which play important roles at different developmental stages. We also found that the MAPK, Hedgehog, and Wnt signaling pathways were significantly involved at different developmental stages. These findings further our understanding of the molecular mecha- nisms that regulate testis development. Our study also demonstrates significant advantages of RNA-seq technology for study- ing transcriptome during development.
Publisher
Science China Press,Springer Nature B.V
Subject
/ Animals
/ Biomedical and Life Sciences
/ DNA, Complementary - chemistry
/ DNA, Complementary - genetics
/ Gene Expression Profiling - methods
/ Gene Expression Regulation, Developmental
/ High-Throughput Nucleotide Sequencing - methods
/ Male
/ Mice
/ Oligonucleotide Array Sequence Analysis
/ Signal Transduction - genetics
/ Testis - growth & development
/ Wnt信号通路
/ 发育过程
/ 基因表达模式
/ 小鼠睾丸
/ 测序
/ 精子发生
/ 组分
/ 转录水平
