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Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755
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Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755
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Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755
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Journal Article
Enhanced butyric acid tolerance and production by Class I heat shock protein-overproducing Clostridium tyrobutyricum ATCC 25755
2017
Overview
The response of
Clostridium tyrobutyricum
to butyric acid stress involves various stress-related genes, and therefore overexpression of stress-related genes can improve butyric acid tolerance and yield. Class I heat shock proteins (HSPs) play an important role in the process of protecting bacteria from sudden changes of extracellular stress by assisting protein folding correctly. The results of quantitative real-time PCR indicated that the Class I HSGs
grpE
,
dnaK
,
dnaJ
,
groEL
,
groES
, and
htpG
were significantly upregulated under butyric acid stress, especially the
dnaK
and
groE
operons. Overexpression of
groESL
and
htpG
could significantly improve the tolerance of
C. tyrobutyricum
to butyric acid, while overexpression of
dnaK
and
dnaJ
showed negative effects on butyric acid tolerance. Acid production was also significantly promoted by increased GroESL expression levels; the final butyric acid and acetic acid concentrations were 28.2 and 38% higher for
C. tyrobutyricum
ATCC 25755/
groESL
than for the wild-type strain. In addition, when fed-batch fermentation was carried out using cell immobilization in a fibrous-bed bioreactor, the butyric acid yield produced by
C. tyrobutyricum
ATCC 25755/
groESL
reached 52.2 g/L, much higher than that for the control. The improved butyric acid yield is probably attributable to the high GroES and GroEL levels, which can stabilize the biosynthetic machinery of
C. tyrobutyricum
under extracellular butyric acid stress.
Publisher
Springer Berlin Heidelberg,Oxford University Press
Subject
/ Acids
/ bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Batch Cell Culture Techniques
/ Bioenergy/Biofuels/Biochemicals - Original Paper
/ Biomedical and Life Sciences
/ Clostridium tyrobutyricum - genetics
/ Clostridium tyrobutyricum - metabolism
/ Gene Expression Regulation, Bacterial
/ Genes
/ Heat-Shock Proteins - genetics
/ Heat-Shock Proteins - metabolism
/ operon
/ Operons
