Journal Article

A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Lee, Howon, Kim, Sungsik, Ryu, Taehoon, Kim, Hwangbeom, Bang, Duhee, Kwon, Sunghoon, Kim, Hyoki

2015

Overview

Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences. One of the biggest bottlenecks in large-scale DNA synthesis is the retrieval of target clonal DNA from high-density sequencing platforms. Here, the authors present a method called ‘Sniper Cloning’ that allows for precise mapping of target clone features and rapid retrieval of targets for full utilization of DNA clones.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Pub. Group
• Subject: 14/19 / 14/63 / 38/77 / 631/1647/1513 / 631/208/514/2254 / 631/337 / Cloning / Deoxyribonucleic acid / DNA / DNA - chemistry / DNA - genetics / High-Throughput Nucleotide Sequencing - methods / Humanities and Social Sciences / Humans / multidisciplinary / Oligonucleotide Array Sequence Analysis / Science / Science (multidisciplinary) / Sequence Analysis, DNA