Journal Article

Conduction pathway for potassium through the Escherichia coli pump KdpFABC

Stokes, David L, Pedersen, Bjørn P, Zhang, Xihui, Hussein, Adel

2025

Overview

Under osmotic stress, bacteria express a heterotetrameric protein complex, KdpFABC, which functions as an ATP-dependent K + pump to maintain intracellular potassium levels. The subunit KdpA belongs to the superfamily of K + transporters and adopts pseudo fourfold symmetry with a membrane-embedded selectivity filter as seen in K + channels. KdpB belongs to the superfamily of P-type ATPases with a conserved binding site for ions within the membrane domain and three cytoplasmic domains that orchestrate ATP hydrolysis via an aspartyl phosphate intermediate. Previous work hypothesized that K + moves parallel to the membrane plane through a 40 Å long tunnel that connects the selectivity filter of KdpA with a canonical binding site in KdpB. In the current work, we have reconstituted KdpFABC into lipid nanodiscs and used cryo-EM to image the wild-type pump under turnover conditions. We present a 2.1 Å structure of the E1~P·ADP conformation, which reveals new features of the conduction pathway. This map shows strong densities within the selectivity filter and at the canonical binding site, consistent with K + bound at each of these sites in this conformation. Many water molecules occupy a vestibule and the proximal end of the tunnel, which becomes markedly hydrophobic and dewetted at the subunit interface. We go on to use ATPase and ion transport assays to assess effects of numerous mutations along this proposed conduction pathway. The results confirm that K + ions pass through the tunnel and support the existence of a low-affinity site in KdpB for releasing these ions to the cytoplasm. Taken together, these data shed new light on the unique partnership between a transmembrane channel and an ATP-driven pump in maintaining the large electrochemical K + gradient essential for bacterial survival.

• Publisher: eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
• Subject: Adenosine Triphosphatases - chemistry / Adenosine Triphosphatases - genetics / Adenosine Triphosphatases - metabolism / Asymmetry / Binding Sites / Cation Transport Proteins - chemistry / Cation Transport Proteins - genetics / Cation Transport Proteins - metabolism / Conduction / cryo-EM / Cryoelectron Microscopy / Cytoplasm / E coli / Escherichia coli - enzymology / Escherichia coli - genetics / Escherichia coli - metabolism / Escherichia coli Proteins - chemistry / Escherichia coli Proteins - genetics / Escherichia coli Proteins - metabolism / Hydrophobicity / Ions / K channel / Kinases / Lipids / Mass spectrometry / membrane transport / Models, Molecular / mutational analysis / Osmotic stress / P-type ATPase / Potassium - metabolism / Protein Conformation / Proteins / Scientific imaging / Structural Biology and Molecular Biophysics