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scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development
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scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development
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Journal Article
scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development
2023
Overview
N
6
-methyladenosine (m
6
A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis and embryogenesis. However, the landscape and function of m
6
A at single cell resolution have not been extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell m
6
A sequencing (scm
6
A-seq) method to simultaneously profile the m
6
A methylome and transcriptome in single oocytes/blastomeres of cleavage-stage embryos. We found that m
6
A deficiency leads to aberrant RNA clearance and consequent low quality of
Mettl3
Gdf9
conditional knockout (cKO) oocytes. We further revealed that m
6
A regulates the translation and stability of modified RNAs in metaphase II (MII) oocytes and during oocyte-to-embryo transition, respectively. Moreover, we observed m
6
A-dependent asymmetries in the epi-transcriptome between the blastomeres of two-cell embryo. scm
6
A-seq thus allows in-depth investigation into m
6
A characteristics and functions, and the findings provide invaluable single-cell resolution resources for delineating the underlying mechanism for gametogenesis and early embryonic development.
Modification of RNA with N6-methyladenosine can regulate RNA metabolism. Here they developed scm
6
A-seq to profile the methylome and transcriptome in single cells, and reveal the functions of m
6
A modification during oocyte maturation and early embryo development.
