Asset Details
Do you wish to reserve the book?
Hakai is required for stabilization of core components of the m6A mRNA methylation machinery
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Hakai is required for stabilization of core components of the m6A mRNA methylation machinery
Do you wish to request the book?
Hakai is required for stabilization of core components of the m6A mRNA methylation machinery
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Hakai is required for stabilization of core components of the m6A mRNA methylation machinery
2021
Overview
N
6
-methyladenosine (m
6
A) is the most abundant internal modification on mRNA which influences most steps of mRNA metabolism and is involved in several biological functions. The E3 ubiquitin ligase Hakai was previously found in complex with components of the m
6
A methylation machinery in plants and mammalian cells but its precise function remained to be investigated. Here we show that Hakai is a conserved component of the methyltransferase complex in
Drosophila
and human cells. In
Drosophila
, its depletion results in reduced m
6
A levels and altered m
6
A-dependent functions including sex determination. We show that its ubiquitination domain is required for dimerization and interaction with other members of the m
6
A machinery, while its catalytic activity is dispensable. Finally, we demonstrate that the loss of Hakai destabilizes several subunits of the methyltransferase complex, resulting in impaired m
6
A deposition. Our work adds functional and molecular insights into the mechanism of the m
6
A mRNA writer complex.
The E3 ligase Hakai can interact with the m
6
A methylation machinery but its function is still unclear. Here, the authors show that Hakai is a conserved component of the m
6
A methyltransferase complex and provide functional and molecular insights into its role in regulating m
6
A levels in
Drosophila
.
