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Multilocus sequence typing analysis and second‐generation sequencing analysis of Salmonella Wandsworth
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Multilocus sequence typing analysis and second‐generation sequencing analysis of Salmonella Wandsworth
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Journal Article
Multilocus sequence typing analysis and second‐generation sequencing analysis of Salmonella Wandsworth
2021
Overview
Background
Salmonella Wandsworth is a rare serotype of Salmonella. This study analyzed the genotyping, genome structure, and molecular biological functions of Salmonella Wandsworth based on the results of multilocus sequence typing and next‐generation sequencing genome assembly analysis.
Methods
Serological typing was performed using the slide‐agglutination method. The micro broth dilution method was used to test antibiotic susceptibility. Multilocus sequence typing (MLST) was used to perform the homology analysis, while the second‐generation sequencing genome analysis was used to analyze the whole genome of the bacteria.
Results
Salmonella Wandsworth is Group Q Salmonella. The MLST of this strain was ST1498. Salmonella Wandsworth was sensitive to antibiotics, such as ceftriaxone, imipenem, chloramphenicol, and colistin, but was resistant to ampicillin, cefalotin, gentamicin, and ciprofloxacin. The second‐generation sequencing results showed that the genome sequence length of the bacteria was 5109457bp. Annotated COG library analysis generated 3,746 corresponding genes. After the comparison with the KEGG library, 1,340 genes, which participate in 19 types of metabolic pathways, were obtained. A total of 249 pathogenic factors and 2 disease islands were predicted. 2 CRISPR sites and 8 Cas sites were predicted. It can be seen from the evolutionary tree that Salmonella Wandsworth MLST1498 and Paratyphi B str.SPB7 are gathered together. We identified one resistance gene, namely, aac(6’)‐Iaa accounting for aminoglycoside resistance.
Conclusion
Salmonella Wandsworth isolated in this study is Salmonella group Q. Consequently, it is necessary to strengthen the understanding of clinical infections of Salmonella Wandsworth and carry out continuous monitoring and research.
Salmonella Wandsworth is a rare serotype of Salmonella. Based on genome sequences, We employed the alignment and assembly‐free (AAF) method to construct a genomic evolutionary tree.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc
Subject
/ Antigens
/ Bacterial Proteins - genetics
/ Bacterial Typing Techniques - methods
/ Colistin
/ CRISPR
/ Farms
/ Genes
/ Genomes
/ High-Throughput Nucleotide Sequencing - methods
/ Homology
/ Humans
/ Imipenem
/ Multilocus sequence typing (MLST)
/ Multilocus Sequence Typing - methods
/ Salmonella - isolation & purification
/ Salmonella Infections - microbiology
/ serotype
