Journal Article

Genome-Wide Identification and Expression Profiling of 2OGD Superfamily Genes from Three Brassica Plants

Jiang, Ding, Cao, Bihao, Chen, Guoju, Chen, Changming, Lei, Jianjun, Li, Guangguang

2021

Overview

The 2-oxoglutarate and Fe(II)-dependent dioxygenase (2OGD) superfamily is the second largest enzyme family in the plant genome, and its members are involved in various oxygenation and hydroxylation reactions. Due to their important biochemical significance in metabolism, a systematic analysis of the plant 2OGD genes family is necessary. Here, we identified 160, 179, and 337 putative 2OGDs from Brassica rapa, Brassica oleracea, and Brassica napus. According to their gene structure, domain, phylogenetic features, function, and previous studies, we also divided 676 2OGDs into three subfamilies: DOXA, DOXB, and DOXC. Additionally, homologous and phylogenetic comparisons of three subfamily genes provided valuable insight into the evolutionary characteristics of the 2OGD genes from Brassica plants. Expression profiles derived from the transcriptome and Genevestigator database exhibited distinct expression patterns of the At2OGD, Br2OGD, and Bo2OGD genes in different developmental stages, tissues, or anatomical parts. Some 2OGD genes showed high expression levels in various tissues, such as callus, seed, silique, and root tissues, while other 2OGD genes were expressed at very low levels in other tissues. Analysis of six Bo2OGD genes in different tissues by qRT-PCR indicated that these genes are involved in the metabolism of gibberellin, which in turn regulates plant growth and development. Our working system analysed 2OGD gene families of three Brassica plants and laid the foundation for further study of their functional characterization.

• Publisher: MDPI AG,MDPI
• Subject: Arabidopsis Proteins - genetics / Biosynthesis / Brassica / Brassica - classification / Brassica - genetics / Brassica - growth & development / Brassica - metabolism / Brassica napus / Brassica napus - genetics / Brassica napus - growth & development / Brassica napus - metabolism / Brassica oleracea / Brassica rapa / Brassica rapa - genetics / Brassica rapa - growth & development / Brassica rapa - metabolism / Callus / Developmental stages / Dioxygenase / Dioxygenases - genetics / DNA repair / Enzymes / Evolution, Molecular / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Plant / Gene families / genes / Genome, Plant / Genomes / Gibberellins / Gibberellins - metabolism / Hydroxylation / Iron / Ketoglutaric acid / Metabolic Networks and Pathways - genetics / Metabolism / Metabolites / Multigene Family / Oxygenation / Phylogenetics / Phylogeny / Plant Development - genetics / plant growth / Plant Proteins - genetics / Proteins / siliques / Transcriptome / Transcriptomes