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Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis
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Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis
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Journal Article
Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis
2020
Overview
Apoptotic cells expressing phosphatidylserine (PS) on their cell surface are directly or indirectly recognized by phagocytes through PS-binding proteins. The PS-binding protein Tim-4 secures apoptotic cells to phagocytes to facilitate the engulfment of apoptotic cells. However, the molecular mechanism by which Tim-4 transduces signals to phagocytes during Tim-4-mediated efferocytosis is incompletely understood. Here, we report that Tim-4 collaborates with Mertk during efferocytosis through a biochemical interaction with Mertk. Proximal localization between the two proteins in phagocytes was observed by immunofluorescence and proximal ligation assays. Physical association between Tim-4 and Mertk, which was mediated by an interaction between the IgV domain of Tim-4 and the fibronectin type-III domain of Mertk, was also detected with immunoprecipitation. Furthermore, the effect of Mertk on Tim-4-mediated efferocytosis was abolished by GST-MertkFnIII, a soluble form of the fibronectin type-III domain of Mertk that disrupts the interaction between Tim-4 and Mertk. Taken together, the results from our study suggest that a physical interaction between Tim-4 and Mertk is necessary for Mertk to enhance efferocytosis mediated by Tim-4.
Publisher
MDPI,MDPI AG
Subject
/ c-Mer Tyrosine Kinase - chemistry
/ c-Mer Tyrosine Kinase - genetics
/ c-Mer Tyrosine Kinase - metabolism
/ Fibronectin Type III Domain - genetics
/ Fibronectin Type III Domain - physiology
/ Humans
/ Membrane Proteins - chemistry
/ Membrane Proteins - genetics
/ Membrane Proteins - metabolism
/ Mertk
/ Mice
/ Tim-4
