Journal Article

Neutrophils negatively control IL-17A-producing γδ T cell frequencies in a contact-dependent manner under physiological conditions

Kasper, Brigitte, Petersen, Frank, Opferman, Joseph T., Yu, Xinhua, Behrends, Jochen, Tchudjin Magatsin, Junie D., Yue, Xiaoyang, Marwitz, Sebastian, Goldmann, Torsten

2025

Overview

In addition to serving as the primary effector cells against infections, neutrophils have been implicated in the regulation of both innate and adaptive immunity. In this study, we aimed to investigate the role of neutrophils in the regulation of the immune system under physiological conditions. The effect of neutrophils on the immune system was examined using neutropenic mice. The interaction between neutrophils and γδ T cells was investigated using an co-culture system. Unexpectedly, we observed an accumulation of γδ T cells in the cervical lymph nodes of neutropenic mice. Transcriptomic analysis revealed that these γδ T cells exhibited unique expression profiles of cell surface molecules and genes involved in defense responses. Further characterization indicated that the accumulated γδ T cells were IL-17 producing CD44 CD62L CD27 memory cells. Additionally, experiments demonstrated that neutrophils could inhibit the function of IL-17A producing γδ T cells by inducing cell death in a contact-dependent manner. This present study demonstrates that neutrophils negatively regulate IL-17 producing γδ T cells under physiological conditions. Given that IL-17A is a critical cytokine for the recruitment of neutrophils to peripheral tissues, our study suggests that the crosstalk between neutrophils and IL-17A producing γδ T cells is a crucial mechanism for maintaining immune homeostasis under physiological conditions.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Adaptive immunity / Animals / Bone marrow / CD27 antigen / CD44 antigen / Cell Communication - immunology / Cell culture / Cell death / Cell surface / Coculture Techniques / Effector cells / Gene expression / Homeostasis / IL-17A / Immune system / Immunology / innate immunity / Interleukin 17 / Interleukin-17 - immunology / Interleukin-17 - metabolism / Intraepithelial Lymphocytes - immunology / L-selectin / Leukocytes (neutrophilic) / Lymph nodes / Lymphocytes / Lymphocytes T / Memory cells / Mice / Mice, Inbred C57BL / Neutropenia / Neutrophils / Neutrophils - immunology / Neutrophils - metabolism / Pathogens / physiological condition / Physiology / Receptors, Antigen, T-Cell, gamma-delta - immunology / Receptors, Antigen, T-Cell, gamma-delta - metabolism / Software / Spleen / Thymus gland / Transcriptomics / γδ T cells