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In vitro cultivation of Amburana cearensis (Allemão) A.C. sm
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In vitro cultivation of Amburana cearensis (Allemão) A.C. sm
In vitro cultivation of Amburana cearensis (Allemão) A.C. sm
Journal Article

In vitro cultivation of Amburana cearensis (Allemão) A.C. sm

2025
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Overview
Umburana is a woody plant from Caatinga with several medicinal uses. Anthropogenic interference in naturally occurring areas and extractivism has put this species at risk of extinction. This study optimizes the multiplication and rooting protocols for the in vitro cultivation of umburana. Two experiments were conducted in a completely randomized design using nodal segments as explants. During the multiplication phase, four culture media, four doses of BAP, and two doses of sucrose were tested, with six replicates. The second experiment focused on rooting, where the plant regulator IBA was tested on explants using three different doses and four durations of permanence, with four replicates. The highest dry biomass was achieved with 30 g. L-1 of sucrose, whereas the greatest fresh biomass, number of buds, and shoots were produced in MS medium with BAP concentrations between 0.5 - 0.6 mg. L-1. To achieve a greater number of roots, 30 days of exposure to IBA at 1 or 2 mg. L-1 is necessary, whereas for producing larger roots, 15 days of exposure to the same IBA concentrations is sufficient. RESUMO: A umburana é uma lenhosa típica da Caatinga que apresenta múltiplos usos, principalmente o potencial medicinal. Interferências antrópicas em áreas de ocorrência natural, aliadas ao extrativismo, tem colocado a espécie em risco de extinção. O objetivo deste estudo foi otimizar o protocolo de multiplicação e enraizamento para o cultivo in vitro de umburana. Dois experimentos foram conduzidos em DIC e segmentos nodais foram utilizados como explantes. Na fase de multiplicação, foram testados quatro meios de cultura, quatro doses de BAP e duas doses de sacarose. No segundo experimento, relativo ao enraizamento, testou-se o regulador vegetal AIB sob os explantes em três doses e quatro períodos de permanência. Identificou-se que maior biomassa seca foi produzida com 30 g.L-1 de sacarose e maior biomassa fresca, número de gemas e brotos em meio MS com concentrações de BAP entre 0,5 - 0,6 mg. L-1. Para maior número de raízes, 30 dias de exposição ao AIB com 1 ou 2 mg. L-1 são necessários, e para a produção de raízes maiores, bastam 15 dias de exposição ao AIB com 1 ou 2 mg. L-1.
Publisher
Universidade Federal de Santa Maria