Journal Article

Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells

Hagio, Hanako, Narantsatsral, Janchiv, Shimizu, Takashi, Terakita, Akihisa, Hibi, Masahiko, Koyanagi, Mitsumasa, Koyama, Wataru, Song, Aysenur Deniz, Matsuda, Koji, Sugihara, Tomohiro, Hosaka, Shiori

2023

Overview

G-protein-coupled receptors (GPCRs) transmit signals into cells depending on the G protein type. To analyze the functions of GPCR signaling, we assessed the effectiveness of animal G-protein-coupled bistable rhodopsins that can be controlled into active and inactive states by light application using zebrafish. We expressed Gq- and Gi/o-coupled bistable rhodopsins in hindbrain reticulospinal V2a neurons, which are involved in locomotion, or in cardiomyocytes. Light stimulation of the reticulospinal V2a neurons expressing Gq-coupled spider Rh1 resulted in an increase in the intracellular Ca 2+ level and evoked swimming behavior. Light stimulation of cardiomyocytes expressing the Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin induced cardiac arrest, and the effect was suppressed by treatment with pertussis toxin or barium, suggesting that Gi/o-dependent regulation of inward-rectifier K + channels controls cardiac function. These data indicate that these rhodopsins are useful for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes.

• Publisher: eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
• Subject: Barium / bistable rhodopsin / Calcium (intracellular) / cardiac contraction / Cardiac muscle / Cardiomyocytes / Cell Biology / Danio rerio / g protein-coupled receptor / G protein-coupled receptors / g protein-coupled rhodopsin / Heart rate / Hindbrain / Kinases / Locomotion / Neurons / Neuroscience / optogenetics / Pertussis / Pertussis toxin / Photoreceptors / Proteins / Rhodopsin / Signal transduction / Swimming behavior / Tools and Resources