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Standardization of large scale production of homologous live attenuated PPR vaccine in India
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Standardization of large scale production of homologous live attenuated PPR vaccine in India
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Journal Article
Standardization of large scale production of homologous live attenuated PPR vaccine in India
2008
Overview
Live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the Indian Veterinary Research Institute, Muktheswar, India. Vero cells between 130-150 passages with six percent foetal calf serum were used for the production of vaccine. The cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic effect was 80%. The vaccine was freezedried in order to maintain the stability of the vaccine. Identity test and titration was performed and the vaccine titre was monitored to be minimum of 10⁵/100 doses. In-house sterility tests and quality control tests using experimental animals and small ruminants were performed. The vacuum and moisture content of the vaccine were also regulated to be within the normal limits.
Publisher
Dordrecht : Springer Netherlands,Springer Netherlands,Springer Nature B.V
Subject
/ Biomedical and Life Sciences
/ Goats
/ India
/ Mice
/ Peste-des-petits-ruminants virus
/ Peste-des-petits-ruminants virus - immunology
/ Sheep
/ TCID
/ Vaccines
/ Vaccines, Attenuated - chemistry
/ Vaccines, Attenuated - immunology
/ Veterinary Medicine/Veterinary Science
/ Viral Vaccines - biosynthesis
/ viruses
/ Zoology
