Journal Article

Molecular Modeling of Lectin-Like Protein from Acacia farnesiana Reveals a Possible Anti-Inflammatory Mechanism in Carrageenan-Induced Inflammation

Cavada, Benildo Sousa, Teixeira, Claudener Souza, Ferreira, Sergio Henrique, Silva-Filho, José Caetano, Gadelha, Tatiane Santi, Delatorre, Plinio, Figueiredo, Jozi Godoy, Batista da Nóbrega, Raphael, Matias da Rocha, Bruno Anderson, Gadelha, Carlos Alberto de Almeida, Abrantes, Vanessa Erika Ferreira

2013

Overview

Acacia farnesiana lectin-like protein (AFAL) is a chitin-binding protein and has been classified as phytohaemagglutinin from Phaseolus vulgaris (PHA). Legume lectins are examples for structural studies, and this family of proteins shows a remarkable conservation in primary, secondary, and tertiary structures. Lectins have ability to reduce the effects of inflammation caused by phlogistic agents, such as carrageenan (CGN). This paper explains the anti-inflammatory activity of AFAL through structural comparison with anti-inflammatory legume lectins. The AFAL model was obtained by molecular modeling and molecular docking with glycan and carrageenan were performed to explain the AFAL structural behavior and biological activity. Pisum sativum lectin was the best template for molecular modeling. The AFAL structure model is folded as a β sandwich. The model differs from template in loop regions, number of β strands and carbohydrate-binding site. Carrageenan and glycan bind to different sites on AFAL. The ability of AFAL binding to carrageenan can be explained by absence of the sixth β-strand (posterior β sheets) and two β strands in frontal region. AFAL can inhibit pathway inflammatory process by carrageenan injection by connecting to it and preventing its entry into the cell and triggers the reaction.

• Publisher: Hindawi Publishing Corporation,John Wiley & Sons, Inc
• Subject: Acacia / Algae / Amino acids / Animals / Anti-Inflammatory Agents - chemistry / Anti-Inflammatory Agents - metabolism / Binding sites / Biological activity / Biomedical research / Carbohydrates / Carrageenan - toxicity / Chitin - chemistry / Crystallography, X-Ray / Inflammation / Inflammation - chemically induced / Inflammation - drug therapy / Inflammation - pathology / Lectins / Legumes / Mice / Migration / Models, Molecular / Molecular Docking Simulation / Permeability / Phytohemagglutinins - chemistry / Phytohemagglutinins - metabolism / Plant lectins / Plant Lectins - administration & dosage / Plant Lectins - chemistry / Plant Lectins - isolation & purification / Plant Lectins - metabolism / Protein Binding / Proteins