Asset Details
Do you wish to reserve the book?
Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model
Do you wish to request the book?
Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model
2009
Overview
The blood–brain barrier (BBB) closely interacts with the neuronal parenchyma in vivo. To replicate this interdependence in vitro, we established a murine coculture model composed of brain endothelial cell (BEC) monolayers with cortical organotypic slice cultures. The morphology of cell types, expression of tight junctions, formation of reactive oxygen species, caspase-3 activity in BECs, and alterations of electrical resistance under physiologic and pathophysiological conditions were investigated. This new BBB model allows the application of techniques such as laser scanning confocal microscopy, immunohistochemistry, fluorescent live cell imaging, and electrical cell substrate impedance sensing in real time for studying the dynamics of BBB function under defined conditions.
Publisher
SAGE Publications,Nature Publishing Group,Sage Publications Ltd
Subject
/ Biological and medical sciences
/ Blood-Brain Barrier - cytology
/ Blood-Brain Barrier - metabolism
/ Coculture Techniques - methods
/ Endothelial Cells - cytology
/ Endothelial Cells - metabolism
/ Membrane Proteins - analysis
/ Membrane Proteins - metabolism
/ Mice
/ Pharmacology. Drug treatments
/ Reactive Oxygen Species - analysis
/ Reactive Oxygen Species - metabolism
/ Tight Junctions - metabolism
/ Vascular diseases and vascular malformations of the nervous system
