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Effect of Energy Substrates on PHB Accumulation of Acidiphilium cryptum DX1-1
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Effect of Energy Substrates on PHB Accumulation of Acidiphilium cryptum DX1-1
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Journal Article
Effect of Energy Substrates on PHB Accumulation of Acidiphilium cryptum DX1-1
2013
Overview
The effect of glucose and elemental sulfur on the growth and PHB accumulation of Acidiphilium cryptum DX1-1 was investigated. Meanwhile, the differential expressions of 19 genes related with PHB accumulation, sulfur metabolism and carbon fixed in heterotrophy, phytotrophy and mixotrophy were studied by RT-qPCR. The results showed that strain DX1-1 could accumulate PHB with sulfur as the energy substance and atmospheric CO₂ as carbon resource. Glucose could improve the growth of strain DX1-1 cultured in medium with sulfur as the energy substance, and almost all the key enzyme-encoding genes related with PHB, sulfur metabolism and carbon fixed were basically up-regulated. PHB polymerase (Arcy_3030), ribulose-bisphosphate carboxylase (Acry_0825), ribulose-phosphate-epimerase (Acry_0022), and cysteine synthase A (Acry_2560) played important role in PHB accumulation, the modified expression of which could influence the PHB yield. With CO₂ as carbon resource, the main initial substance of PHB accumulation for strain DX1-1 was acetyl-CoA, instead of acetate with the glucose as the carbon resource. Because of accumulating PHB by fixed atmospheric CO₂ while independent of light, A. cryptum DX1-1 may have specifically potential in production of PHB.
Publisher
Springer-Verlag,Springer US,Springer Nature B.V
Subject
/ acetates
/ Biomedical and Life Sciences
/ carbon
/ cysteine
/ energy
/ genes
/ genetics
/ Glucose
/ Hydroxybutyrates - metabolism
/ Metabolic Networks and Pathways
/ Metabolic Networks and Pathways - genetics
/ Plastics
/ Real-Time Polymerase Chain Reaction
/ ribulose-bisphosphate carboxylase
/ Sulfur
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