Journal Article

Astaxanthin alleviates PM2.5-induced cardiomyocyte injury via inhibiting ferroptosis

Zhang, Yadong, Ren, Jingyi, Guo, Zihao, Zhu, Siqi, Pei, Huanting, Zuo, Jinshi, Ma, Yuxia, Sun, Xiaoya, Wen, Rui, Yin, Bowen, Wang, Ziyi

2023

Overview

Background Long-term exposure of humans to air pollution is associated with an increasing risk of cardiovascular diseases (CVDs). Astaxanthin (AST), a naturally occurring red carotenoid pigment, was proved to have multiple health benefits. However, whether or not AST also exerts a protective effect on fine particulate matter (PM 2.5 )-induced cardiomyocyte damage and its underlying mechanisms remain unclear. Methods In vitro experiments, the H9C2 cells were subjected to pretreatment with varying concentrations of AST, and then cardiomyocyte injury model induced by PM 2.5 was established. The cell viability and the ferroptosis-related proteins expression were measured in different groups. In vivo experiments, the rats were pretreated with different concentrations of AST for 21 days. Subsequently, a rat model of myocardial PM 2.5 injury was established by intratracheal instillation every other day for 1 week. The effects of AST on myocardial tissue injury caused by PM 2.5 indicating by histological, serum, and protein analyses were examined. Results AST significantly ameliorated PM 2.5 -induced myocardial tissue injury, inflammatory cell infiltration, the release of inflammatory factors, and cardiomyocyte H9C2 cell damage. Mechanistically, AST pretreatment increased the expression of SLC7A11, GPX4 and down-regulated the expression of TfR1, FTL and FTH1 in vitro and in vivo. Conclusions Our study suggest that ferroptosis plays a significant role in the pathogenesis of cardiomyocyte injury induced by PM 2.5 . AST may serve as a potential therapeutic agent for mitigating cardiomyocyte injury caused by PM 2.5 through the inhibition of ferroptosis.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Air pollution / Astaxanthin / Biochemistry / Bioengineering / Bioinformatics / Biological and Medical Physics / Biomedical and Life Sciences / Biophysics / Biotechnology / Cardiomyocyte injury / Cardiomyocytes / Cardiovascular diseases / Cell Biology / Cell death / Cell viability / Ferroptosis / Gene expression / Inflammation / Kinases / Laboratory animals / Life Sciences / Ligands / Molecular Medicine / Olive oil / Outdoor air quality / Particulate matter / PM2.5 / Research Article / Software / Trachea / Traditional Chinese medicine / Tumor necrosis factor-TNF