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Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
by
Sandmann, Gerhard
, Zhu, Changfu
, Naqvi, Shaista
, Capell, Teresa
, Christou, Paul
, Farré, Gemma
in
4-Hydroxyphenylpyruvate Dioxygenase - genetics
/ 4-Hydroxyphenylpyruvate Dioxygenase - metabolism
/ Animal Genetics and Genomics
/ Arabidopsis
/ Arabidopsis - enzymology
/ Arabidopsis - genetics
/ biochemical pathways
/ biofortification
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Biotechnology
/ Biotechnology - methods
/ Brief Communication
/ clones
/ complementary DNA
/ corn
/ Fundamental and applied biological sciences. Psychology
/ gamma-tocopherol
/ gamma-Tocopherol - metabolism
/ gene expression
/ Gene Expression Regulation, Plant
/ Genetic Engineering
/ Genetic Engineering - methods
/ Genetic technics
/ human health
/ Humans
/ isomers
/ Life Sciences
/ Metabolic engineering
/ Methods. Procedures. Technologies
/ methyltransferases
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Molecular Medicine
/ Plant Genetics and Genomics
/ Plants, Genetically Modified - enzymology
/ Plants, Genetically Modified - genetics
/ seeds
/ substrate specificity
/ tocopherols
/ Transgenic animals and transgenic plants
/ Transgenic maize
/ transgenic plants
/ Transgenics
/ Up-Regulation
/ vitamin E
/ Zea mays - enzymology
/ Zea mays - genetics
2011
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Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
by
Sandmann, Gerhard
, Zhu, Changfu
, Naqvi, Shaista
, Capell, Teresa
, Christou, Paul
, Farré, Gemma
in
4-Hydroxyphenylpyruvate Dioxygenase - genetics
/ 4-Hydroxyphenylpyruvate Dioxygenase - metabolism
/ Animal Genetics and Genomics
/ Arabidopsis
/ Arabidopsis - enzymology
/ Arabidopsis - genetics
/ biochemical pathways
/ biofortification
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Biotechnology
/ Biotechnology - methods
/ Brief Communication
/ clones
/ complementary DNA
/ corn
/ Fundamental and applied biological sciences. Psychology
/ gamma-tocopherol
/ gamma-Tocopherol - metabolism
/ gene expression
/ Gene Expression Regulation, Plant
/ Genetic Engineering
/ Genetic Engineering - methods
/ Genetic technics
/ human health
/ Humans
/ isomers
/ Life Sciences
/ Metabolic engineering
/ Methods. Procedures. Technologies
/ methyltransferases
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Molecular Medicine
/ Plant Genetics and Genomics
/ Plants, Genetically Modified - enzymology
/ Plants, Genetically Modified - genetics
/ seeds
/ substrate specificity
/ tocopherols
/ Transgenic animals and transgenic plants
/ Transgenic maize
/ transgenic plants
/ Transgenics
/ Up-Regulation
/ vitamin E
/ Zea mays - enzymology
/ Zea mays - genetics
2011
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Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
by
Sandmann, Gerhard
, Zhu, Changfu
, Naqvi, Shaista
, Capell, Teresa
, Christou, Paul
, Farré, Gemma
in
4-Hydroxyphenylpyruvate Dioxygenase - genetics
/ 4-Hydroxyphenylpyruvate Dioxygenase - metabolism
/ Animal Genetics and Genomics
/ Arabidopsis
/ Arabidopsis - enzymology
/ Arabidopsis - genetics
/ biochemical pathways
/ biofortification
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Biotechnology
/ Biotechnology - methods
/ Brief Communication
/ clones
/ complementary DNA
/ corn
/ Fundamental and applied biological sciences. Psychology
/ gamma-tocopherol
/ gamma-Tocopherol - metabolism
/ gene expression
/ Gene Expression Regulation, Plant
/ Genetic Engineering
/ Genetic Engineering - methods
/ Genetic technics
/ human health
/ Humans
/ isomers
/ Life Sciences
/ Metabolic engineering
/ Methods. Procedures. Technologies
/ methyltransferases
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Molecular Medicine
/ Plant Genetics and Genomics
/ Plants, Genetically Modified - enzymology
/ Plants, Genetically Modified - genetics
/ seeds
/ substrate specificity
/ tocopherols
/ Transgenic animals and transgenic plants
/ Transgenic maize
/ transgenic plants
/ Transgenics
/ Up-Regulation
/ vitamin E
/ Zea mays - enzymology
/ Zea mays - genetics
2011
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Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
Journal Article
Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
2011
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Overview
The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to q-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constitutively expressed in corn to further characterize the pathway and increase the kernel tocopherol content. Transgenic kernels contained up to 3 times as much c-tocopherol as their wild type counterparts whereas other tocopherol isomers remained undetectable. Biofortification by metabolic engineering offers a sustainable alternative to vitamin E supplementation for the improvement of human health.
Publisher
Springer Verlag,Dordrecht : Springer Netherlands,Springer Netherlands,Springer
Subject
4-Hydroxyphenylpyruvate Dioxygenase - genetics
/ 4-Hydroxyphenylpyruvate Dioxygenase - metabolism
/ Animal Genetics and Genomics
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ clones
/ corn
/ Fundamental and applied biological sciences. Psychology
/ gamma-Tocopherol - metabolism
/ Gene Expression Regulation, Plant
/ Genetic Engineering - methods
/ Humans
/ isomers
/ Methods. Procedures. Technologies
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Plants, Genetically Modified - enzymology
/ Plants, Genetically Modified - genetics
/ seeds
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