Asset Details
Do you wish to reserve the book?
The existence of aspolin and its trimethylamine-N-oxide demethylating activity in the muscle of freshwater fish
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
The existence of aspolin and its trimethylamine-N-oxide demethylating activity in the muscle of freshwater fish
Do you wish to request the book?
The existence of aspolin and its trimethylamine-N-oxide demethylating activity in the muscle of freshwater fish
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
The existence of aspolin and its trimethylamine-N-oxide demethylating activity in the muscle of freshwater fish
2005
Overview
: Aspolin is a polyaspartic acid‐like protein, which is originally isolated from walleye pollack Theragra chalcogramma muscle as trimethylamine‐N‐oxide (TMAO) demethylase. Although carp Cyprinus carpio muscle contains a trace amount of the enzyme substrate, TMAO, aspolin can be extracted and purified by acid treatment, successive chromatographies and polyacrylamide gel electrophoresis, and has twice the amount of that in walleye pollack muscle. Carp aspolin showed a low enzymatic activity in the presence of Fe2+ and reductants, and its Km value (100 mM) to TMAO was extremely high. It was a thermostable protein and had an unfolded conformation. The amino acid sequence of carp aspolin 1 deduced from cDNA revealed that it contained a long Asp polymer, an uninterrupted stretch of 138 Asp residues, followed by four amino acid residues, His‐Glu‐Glu‐Leu, in C‐terminus. The chain length was shorter by 42 Asp residues than that of its walleye pollack counterpart.
Publisher
Blackwell Science Pty,Springer Nature B.V
