Journal Article

GDP-L-fucose is required for boundary definition in plants

Blein, Thomas, Goncalves, Beatriz, Maugarny-Calès, Aude, Groupe de Recherche Angevin en Economie et Management (GRANEM) ; Université d'Angers (UA)-AGROCAMPUS OUEST-Institut National de l'Horticulture et du Paysage, Mouille, Gregory, Cortizo, Millan, Laufs, Patrick, P, Hasson, Alice, Agence National de la Recherche grant LEAFNET [ANR-12-PDOC-0003] ; Agence National de la Recherche grant MORPHOLEAF [ANR-10-BLAN-1614] ; Fundacion Alfonso Martin Escudero ; Labex Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], Borrega, Néro, N, Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Adroher, Bernard, B, ANR-10-LABX-0040,SPS,Saclay Plant Sciences, Gineau, Emilie, Arnaud, Nicolas

2017

Overview

The CUP-SHAPED COTYLEDON (CUC) transcription factors control plant boundary formation, thus allowing the emergence of novel growth axes. While the developmental roles of the CUC genes in different organs and across species are well characterized, upstream and downstream events that contribute to their function are still poorly understood. To identify new players in this network, we performed a suppressor screen of CUC2g-m4, a line overexpressing CUC2 that has highly serrated leaves. We identified a mutation that simplifies leaf shape and affects MURUS1 (MUR1), which is responsible for GDP-L-fucose production. Using detailed morphometric analysis, we show that GDP-L-fucose has an essential role in leaf shape acquisition by sustaining differential growth at the leaf margins. Accordingly, reduced CUC2 expression levels are observed in mur1 leaves. Furthermore, genetic analyses reveal a conserved role for GDPL- fucose in different developmental contexts where it contributes to organ separation in the same pathway as CUC2. Taken together, our results reveal that GDP-L-fucose is necessary for proper establishment of boundary domains in various developmental contexts.

• Publisher: Oxford University Press (OUP),CCSD,Oxford University Press
• Subject: Arabidopsis - genetics / Arabidopsis - growth & development / Arabidopsis - metabolism / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Gene Expression Regulation, Developmental / Gene Expression Regulation, Plant / Growth and Development / Guanosine Diphosphate Fucose - genetics / Guanosine Diphosphate Fucose - metabolism / Life Sciences / Mutation / Plant Leaves - genetics / Plant Leaves - growth & development / Plant Leaves - metabolism / Research Papers / Transcription Factors - genetics / Transcription Factors - metabolism / Vegetal Biology
• ISBN: 0004183656000