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Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae
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Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae
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Journal Article
Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae
2016
Overview
The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831 bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (
pelu112
). The
pelu112
gene was predicted to encode a fusion of
thymidylate kinase
(
tmk
) and
dUTP diphosphatase
(
dut
). Phylogenetic analysis indicated that baculoviruses have independently acquired
tmk
and
dut
several times during their evolution. Two homologs of the
tmk-dut
fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks
tmk
and
dut
. The recombinant baculoviruses produced viral DNA, virus progeny and some viral proteins earlier during
in vitro
infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these
tmk-dut
fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 49/23
/ Animals
/ DNA, Viral - isolation & purification
/ Enzymes
/ Genes
/ Genetic Vectors - metabolism
/ Genomes
/ Humanities and Social Sciences
/ Kinases
/ Microscopy, Electron, Scanning
/ Nucleopolyhedrovirus - classification
/ Nucleopolyhedrovirus - genetics
/ Nucleopolyhedrovirus - physiology
/ Nucleoside-Phosphate Kinase - chemistry
/ Nucleoside-Phosphate Kinase - genetics
/ Nucleoside-Phosphate Kinase - metabolism
/ Open Reading Frames - genetics
/ Pyrophosphatases - chemistry
/ Pyrophosphatases - metabolism
/ Science
/ Tandem Repeat Sequences - genetics
/ Viruses
