Journal Article

LANDSCAPE GENOMICS OF POPULUS TRICHOCARPA: THE ROLE OF HYBRIDIZATION, LIMITED GENE FLOW, AND NATURAL SELECTION IN SHAPING PATTERNS OF POPULATION STRUCTURE

Grassa, Christopher J., Douglas, Carl J., Geraldes, Armando, McKown, Athena D., Cronk, Quentin C. B., Guy, Robert D., Farzaneh, Nima, Mansfield, Shawn D.

2014

Overview

Populus trichocarpa is an ecologically important tree across western North America. We used a large population sample of 498 accessions over a wide geographical area genotyped with a 34K Populus SNP array to quantify geographical patterns of genetic variation in this species (landscape genomics). We present evidence that three processes contribute to the observed patterns: (1) introgression from the sister species P. balsamifera, (2) isolation by distance (IBD), and (3) natural selection. Introgression was detected only at the margins of the species' distribution. IBD was significant across the sampled area as a whole, but no evidence of restricted gene flow was detected in a core of drainages from southern British Columbia (BC). We identified a large number of FST outliers. Gene Ontology analyses revealed that FST outliers are overrepresented in genes involved in circadian rhythm and response to red/far-red light when the entire dataset is considered, whereas in southern BC heat response genes are overrepresented. We also identified strong correlations between geoclimate variables and allele frequencies at FST outlier loci that provide clues regarding the selective pressures acting at these loci.

• Publisher: Blackwell Publishing Ltd,Society for the Study of Evolution,Oxford University Press
• Subject: Adaptation / Admixtures / Biological variation / Circadian rhythms / Datasets / Evolution / Evolutionary genetics / FST outliers / Gene Flow / Gene frequency / Gene loci / Genes / Genes, Plant / Genetic diversity / Genetic loci / Genetic Variation / Genetics, Population / Genomics / Genotype & phenotype / Geographical distribution / Hybridization / Hybridization, Genetic / isolation by distance / Loci / Natural selection / Nonnative species / Outliers / Outliers (statistics) / Phenotype / Phenotypic traits / Phylogeography / Plant populations / Polymorphism, Single Nucleotide / Population genetics / Population structure / Populus / Populus - classification / Populus - genetics / Populus - physiology / Populus balsamifera / Populus trichocarpa / red/far-red / Selection, Genetic / Sibling species / Single-nucleotide polymorphism / SNPs / Trees