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Structural and functional insights into the LBD family involved in abiotic stress and flavonoid synthases in Camellia sinensis
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Structural and functional insights into the LBD family involved in abiotic stress and flavonoid synthases in Camellia sinensis
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Journal Article
Structural and functional insights into the LBD family involved in abiotic stress and flavonoid synthases in Camellia sinensis
2019
Overview
Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors that play a crucial role in growth and development, as well as metabolic processes. However, knowledge of the function of LBD proteins in
Camellia sinensis
is limited, and no systematic investigations of the LBD family have been reported. In this study, we identified 54 LBD genes in
Camellia sinensis
. The expression patterns of
CsLBDs
in different tissues and their transcription responses to exogenous hormones and abiotic stress were determined by RNA-seq, which showed that CsLBDs may have diverse functions. Analysis of the structural gene promoters revealed that the promoters of
CsC4H
,
CsDFR
and
CsUGT84A
, the structural genes involved in flavonoid biosynthesis, contained LBD recognition binding sites. The integrative analysis of
CsLBD
expression levels and metabolite accumulation also suggested that CsLBDs are involved in the regulation of flavonoid synthesis. Among them, CsLOB_3, CsLBD36_2 and CsLBD41_2, localized in the nucleus, were selected for functional characterization. Yeast two-hybrid assays revealed that CsLBD36_2 and CsLBD41_2 have self-activation activities, and CsLOB_3 and CsLBD36_2 can directly bind to the
cis
-element and significantly increase the activity of the
CsC4H
,
CsDFR
and
CsUGT84A
promoter. Our results present a comprehensive characterization of the 54
CsLBDs
in
Camellia sinensis
and provide new insight into the important role that
CsLBDs
play in abiotic and flavonoid biosynthesis.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/39
/ Camellia sinensis - cytology
/ Camellia sinensis - genetics
/ Camellia sinensis - metabolism
/ Camellia sinensis - physiology
/ Gene Expression Regulation, Plant
/ Hormones
/ Humanities and Social Sciences
/ Intracellular Space - metabolism
/ Molecular Sequence Annotation
/ Promoter Regions, Genetic - genetics
/ RNA
/ Science
/ Structure-function relationships
/ Transcription Factors - chemistry
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Yeast
