Journal Article

Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data Measured between 3.6 °C and 25.4 °C for the Fab Fragment of NISTmAb

Hudgens, Jeffrey W., Bou-Assaf, George M., Pandey, Ratnesh, Zhang, Hui-Min, Li, Sheng, Rouse, Jason C., Deredge, Daniel, Espada, Alfonso, Venable, John, Urata, Sarah, Steckler, Caitlin, Chandramohan, Arun, Brock, Ansgar, Anderson, Kyle W., Walters, Benjamin T., Carroll, James A., Leurs, Ulrike, Srinivasan Anand, Ganesh, Rand, Kasper D., Lu, Xiaojun, Hageman, Tyler S., Wintrode, Patrick L., Lambris, John D., Chalmers, Michael J., Huang, Richard Y.-C., Sperry, Justin B., Zhang, Jennifer, Papanastasiou, Malvina, Park, Inhee, Gallagher, Elyssia S., Harguindey, Eduardo, Nirudodhi, Sasidhar N., Chen, Guodong, Weis, David D., Karageorgos, Ioannis, Al-Naqshabandi, Mohammed A.

2019

Overview

The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the FabFragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 oC and 25.4 oC for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and Q-TOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.

• Publisher: National Institute of Standards and Technology,Superintendent of Documents,[Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology
• Subject: Amides / Analytical chemistry / Centroids (Geometry) / Chemical bonds / Chromatography / Deuterium / Hydrogen / Ions / Laboratories / Mass spectrometry / Measurement / Methods / Monoclonal antibodies / New technology / Peptides / Proteins / Proteomics / Reagents / Reference materials / Scientific imaging / Spectra / Statistics / Submerging