Journal Article

IL-10R inhibition reprograms tumor-associated macrophages and reverses drug resistance in multiple myeloma

Diedrich, Camila, Maksimos, Mina, Wang, Shaomeng, Vij, Ravi, Kelley, Sarah, Zhou, Haibin, Alhallak, Kinan, Muz, Barbara, Bash, Hannah, Corradini, Stefan, Park, Chaelee, Bender, Diane E., Azab, Abdel Kareem, Lubben, Berit, Shokeen, Monica, Azab, Feda, Fiala, Mark, Sun, Jennifer, Miari, Katerina E., Asare, Obed, Chen, Yixuan, Adebayo, Ola, Williams, Mark T. S.

2024

Overview

Multiple myeloma (MM) is the cancer of plasma cells within the bone marrow and remains incurable. Tumor-associated macrophages (TAMs) within the tumor microenvironment often display a pro-tumor phenotype and correlate with tumor proliferation, survival, and therapy resistance. IL-10 is a key immunosuppressive cytokine that leads to recruitment and development of TAMs. In this study, we investigated the role of IL-10 in MM TAM development as well as the therapeutic application of IL-10/IL-10R/STAT3 signaling inhibition. We demonstrated that IL-10 is overexpressed in MM BM and mediates M2-like polarization of TAMs in patient BM, 3D co-cultures in vitro, and mouse models. In turn, TAMs promote MM proliferation and drug resistance, both in vitro and in vivo. Moreover, inhibition of IL-10/IL-10R/STAT3 axis using a blocking IL-10R monoclonal antibody and STAT3 protein degrader/PROTAC prevented M2 polarization of TAMs and the consequent TAM-induced proliferation of MM, and re-sensitized MM to therapy, in vitro and in vivo. Therefore, our findings suggest that inhibition of IL-10/IL-10R/STAT3 axis is a novel therapeutic strategy with monotherapy efficacy and can be further combined with current anti-MM therapy, such as immunomodulatory drugs, to overcome drug resistance. Future investigation is warranted to evaluate the potential of such therapy in MM patients.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/1 / 13/106 / 13/21 / 13/31 / 59/5 / 631/67/327 / 64/60 / 692/699/1541/1990/804 / Animal models / Animals / Biodegradation / Bone cancer / Bone marrow / Cancer Research / Cell Line, Tumor / Cell proliferation / Cell Proliferation - drug effects / Critical Care Medicine / Drug development / Drug resistance / Drug Resistance, Neoplasm / Hematology / Humans / Immunomodulation / Immunosuppressive agents / In vivo methods and tests / Intensive / Interleukin 10 / Internal Medicine / Macrophages / Medicine / Medicine & Public Health / Mice / Monoclonal antibodies / Multiple myeloma / Multiple Myeloma - drug therapy / Multiple Myeloma - pathology / Oncology / Phenotypes / Plasma cells / Polarization / Receptors, Interleukin-10 - antagonists & inhibitors / Receptors, Interleukin-10 - metabolism / Signal Transduction - drug effects / Stat3 protein / STAT3 Transcription Factor - antagonists & inhibitors / STAT3 Transcription Factor - metabolism / Therapy / Tumor microenvironment / Tumor Microenvironment - drug effects / Tumor-Associated Macrophages - drug effects / Tumor-Associated Macrophages - immunology / Tumor-Associated Macrophages - metabolism / Tumors