Journal Article

Hampshire Sheep as a Large-Animal Model for Cochlear Implantation

Cheng, Yew Song, Chern, Alexander, Nakajima, Hideko Heidi, Olson, Elizabeth S., Lang, Jeffrey H., Zhou, Chaoqun, Waring, Nicholas A., Vilarello, Brandon J.

2024

Overview

Background Sheep have been proposed as a large-animal model for studying cochlear implantation. However, prior sheep studies report that the facial nerve (FN) obscures the round window membrane (RWM), requiring FN sacrifice or a retrofacial opening to access the middle-ear cavity posterior to the FN for cochlear implantation. We investigated surgical access to the RWM in Hampshire sheep compared to Suffolk-Dorset sheep and the feasibility of Hampshire sheep for cochlear implantation via a facial recess approach. Methods Sixteen temporal bones from cadaveric sheep heads (ten Hampshire and six Suffolk-Dorset) were dissected to gain surgical access to the RWM via an extended facial recess approach. RWM visibility was graded using St. Thomas’ Hospital (STH) classification. Cochlear implant (CI) electrode array insertion was performed in two Hampshire specimens. Micro-CT scans were obtained for each temporal bone, with confirmation of appropriate electrode array placement and segmentation of the inner ear structures. Results Visibility of the RWM on average was 83% in Hampshire specimens and 59% in Suffolk-Dorset specimens ( p  = 0.0262). Hampshire RWM visibility was Type I (100% visibility) for three specimens and Type IIa (> 50% visibility) for seven specimens. Suffolk-Dorset RWM visibility was Type IIa for four specimens and Type IIb (< 50% visibility) for two specimens. FN appeared to course more anterolaterally in Suffolk-Dorset specimens. Micro-CT confirmed appropriate CI electrode array placement in the scala tympani without apparent basilar membrane rupture. Conclusions Hampshire sheep appear to be a suitable large-animal model for CI electrode insertion via an extended facial recess approach without sacrificing the FN. In this small sample, Hampshire specimens had improved RWM visibility compared to Suffolk-Dorset. Thus, Hampshire sheep may be superior to other breeds for ease of cochlear implantation, with FN and facial recess anatomy more similar to humans.

• Publisher: Springer US,Springer Nature B.V
• Subject: Animal models / Animals / Arrays / Bones / Cadavers / Cochlea / Cochlear Implantation - methods / Cochlear Implants / Computed tomography / Electrodes / Facial nerve / Hogs / Inner ear / Medical imaging / Medicine / Medicine & Public Health / Models, Animal / Neurobiology / Neurosciences / Open source software / Original / Original Article / Otorhinolaryngology / Public domain / Round Window, Ear - anatomy & histology / Round Window, Ear - surgery / Sheep / Temporal Bone