Journal Article

Performance, Egg Quality, and Intestinal Morphology of Laying Hens Fed High-Fiber Diets with or Without Stimbiotic Supplementation

Kaneko, Isabelle Naemi, Lima, Matheus Ramalho de, Costa, Fernando Guilherme Perazzo, Rousseau, Xavière, Lima, Adiel Vieira de, Lima, Amanda Fabrício Dantas de, Cavalcante, Danilo Teixeira, Vieira, Danilo Vargas Gonçalves, Silva, Edijanio Galdino da, Nascimento, Germano Augusto Jerônimo do, Souza, Paloma Eduarda Lopes de, Nascimento, Carlos Henrique do, Guerra, Ricardo Romão

2026

Overview

Moderately fermentable dietary fiber (with different crude fiber [CF] levels), especially when combined with stimbiotic (STB) supplementation, can enhance intestinal health, nutrient utilization, and overall performance in laying hens, although effects depend on fiber type, level, and diet composition. To investigate this, 1200 Bovans White laying hens (32 weeks old) were assigned to a 2 × 6 factorial experiment with two levels of supplementation (without or with 0.01% STB) and six dietary fiber treatments: Control (commercial diet), Corn–soybean, 75:25 wheat–corn, 50:50 wheat–corn, 25:75 wheat–corn, and Corn–soybean. The study spanned five 28-day periods, evaluating productive performance, egg quality, and intestinal morphology. Dietary fiber levels significantly improved feed intake (p = 0.0029), egg production (p < 0.0001), egg mass (p < 0.0001), feed conversion (p < 0.0001), and intestinal structure (p ≤ 0.05), while STB alone had limited effects. Hens fed 75:25 and 50:50 wheat–corn diets consumed more feed, and the highest egg production and mass were observed in layers receiving Control, 75:25 wheat–corn, and Corn–soybean diets. Egg quality benefited from the fiber–STB interaction, producing heavier eggs with higher yolk pigmentation (p ≤ 0.05), thicker shells (p ≤ 0.05), and specific gravity (p ≤ 0.05). STB supplementation increased jejunal villus width (p = 0.0001) and absorptive area (p = 0.0063), whereas fiber type affected ileal villus width (p = 0.0025) and absorptive area (p = 0.0156). Fiber–STB interaction influences the duodenum villus width (p = 0.0106), crypt depth (p = 0.0011), villus-to-crypt ratio (p = 0.0058), and absorptive area (p = 0.0086), and ileum villus width (p = 0.0011), crypt depth (p = 0.0058), and absorptive area (p = 0.0086). In conclusion, the use of 0.01% STB in diets with high crude fiber levels (25:75 and 75:25 wheat–corn ratios) improves performance, egg quality, and intestinal health in laying hens.

• Publisher: MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI)
• Subject: Animal welfare / Costs (Law) / Diet / Dietary fiber / egg production / egg quality / Eggs / Enzymes / Feeds / fermentable fiber / gut health / Livestock farms / Microbiota / Morphology / Nutrition research / Poultry industry / Soybeans / wheat bran / xylanase