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Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture
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Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture
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Journal Article
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture
2014
Overview
Insulin-producing cells (IPCs) derived from human pluripotent stem cells (hPSCs) may be useful in cell therapy and drug discovery for diabetes. Here, we examined various growth factors and small molecules including those previously reported to develop a robust differentiation method for induction of mature IPCs from hPSCs. We established a protocol that induced PDX1-positive pancreatic progenitor cells at high efficiency and further induced mature IPCs by treatment with forskolin, dexamethasone, Alk5 inhibitor II and nicotinamide in 3D culture. The cells that differentiated into INSULIN-positive and C-PEPTIDE-positive cells secreted insulin in response to glucose stimulation, indicating a functional IPC phenotype. We also found that this method was applicable to different types of hPSCs.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/31
/ 13/51
/ 38/77
/ 38/88
/ 45/90
/ 82
/ Humanities and Social Sciences
/ Humans
/ Insulin-Secreting Cells - cytology
/ Insulin-Secreting Cells - metabolism
/ Pluripotent Stem Cells - cytology
/ Pluripotent Stem Cells - metabolism
/ Protein-Serine-Threonine Kinases - antagonists & inhibitors
/ Protein-Serine-Threonine Kinases - metabolism
/ Receptors, Transforming Growth Factor beta - antagonists & inhibitors
/ Receptors, Transforming Growth Factor beta - metabolism
/ Science
