Journal Article

Extracellular Vesicles Profile and Risk of Venous Thromboembolism in Patients with Diffuse Large B-Cell Lymphoma

Masic, Jelena Vladicic, Antic, Darko, Gran, Charlotte, Mihaljevic, Biljana, Dukic, Nikolina, Milic, Natasa, Fareed, Jawed, Ivanovic, Jelena, Antovic, Jovan, Kozarac, Sofija, Otasevic, Vladimir, Vukovic, Vojin

2025

Overview

Thrombosis is a common complication in cancer patients, with a substantial impact on morbidity and mortality. Diffuse large B-cell lymphoma (DLBCL) and other aggressive lymphomas carry a high venous thromboembolism (VTE) risk. Extracellular vesicles (EVs) have gained attention in recent research as a new potential biomarker for VTE development. To determine the profile and association of EVs with VTE in patients with DLBCL, we conducted a prospective cohort study on 62 patients diagnosed with DLBCL. A total of 11 patients (17.7%) developed VTE. The concentrations of platelet-derived EVs (PEVs), E-selectin+ EVs, P-selectin+ EVs, tissue factor (TF)-positive/CD20+ EVs, TF−/CD19+ EVs, TF−/CD45+ EVs, and TF−/CD20+ EVs were significantly higher in DLBCL patients compared to healthy controls. In contrast, the concentration of TF− PEVs was significantly lower in DLBCL patients compared to healthy controls. No statistically significant differences were observed in the concentrations of the EV profiles among the DLBCL patients with and without VTE. Using Cox regression analysis, we found that none of the observed EV populations demonstrated an association with overall survival (OS). In conclusion, patients with DLBCL have elevated concentrations of distinct EV populations—in particular, PEVs, E-selectin EVs, P-selectin EVs, TF+/CD20+ EVs, and TF− DLBCL/B-cell EVs (CD19, CD20, CD45)—compared to healthy controls. DLBCL patients exhibit a specific EV profile, which is not significantly related to the risk of VTE and OS outcomes. Our data provide an intriguing insight into EV profiles in patients with DLBCL. Additional research is needed to elucidate these findings further.

• Publisher: MDPI AG,MDPI
• Subject: Adult / Aged / Aged, 80 and over / Cancer / Cancer therapies / Care and treatment / Chemotherapy / E-Selectin - metabolism / Extracellular vesicles / Extracellular Vesicles - metabolism / Female / Health aspects / Hematology / Humans / Localization / Lymphoma / Lymphoma, Large B-Cell, Diffuse - complications / Lymphoma, Large B-Cell, Diffuse - metabolism / Lymphoma, Large B-Cell, Diffuse - pathology / Male / Medical research / Medicine, Experimental / MicroRNAs / Middle Aged / Non-Hodgkin's lymphomas / P-Selectin - metabolism / Patients / Physiology / Prospective Studies / Risk Factors / Thromboembolism / Thromboplastin - metabolism / Thrombosis / Veins & arteries / Venous Thromboembolism - etiology / Venous Thromboembolism - metabolism