Asset Details
Do you wish to reserve the book?
Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos
Do you wish to request the book?
Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos
2020
Overview
Specification of embryonic lineages is an important question in the field of early development. Numerous studies analyzed the expression patterns of the candidate transcripts and proteins in humans and mice and clearly determined the markers of each lineage. To overcome the limitations of human and mouse embryos, the expression of the marker transcripts in each cell has been investigated using in vivo embryos in pigs. In vitro produced embryos are more accessible, can be rapidly processed with low cost. Therefore, we analyzed the characteristics of lineage markers and the effects of the DAB2 gene (trophectoderm marker) in in vitro fertilized porcine embryos. We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. Marker transcripts showed diverse patterns of expression, and each embryonic lineage could be identified with localization of marker proteins. In DAB2-shRNA vectors injected embryos, HNF4A and PDGFRA were upregulated. DAB2 protein level was lower in shRNA-injected embryos without significant differences. Our results will contribute to understanding of the mechanisms of embryonic lineage specification in pigs.
Publisher
MDPI AG,MDPI
Subject
Adaptor Proteins, Vesicular Transport - antagonists & inhibitors
/ Adaptor Proteins, Vesicular Transport - genetics
/ Adaptor Proteins, Vesicular Transport - metabolism
/ Animals
/ Ectoderm - growth & development
/ Eggs
/ Embryos
/ Female
/ Gene Expression Regulation, Developmental
/ Hepatocyte Nuclear Factor 4 - genetics
/ Hepatocyte Nuclear Factor 4 - metabolism
/ Hogs
/ Male
/ Mammals
/ Proteins
/ Receptor, Platelet-Derived Growth Factor alpha - genetics
/ Receptor, Platelet-Derived Growth Factor alpha - metabolism
/ RNA, Small Interfering - genetics
/ RNA, Small Interfering - metabolism
/ Swine
