Journal Article

Murine Inner Cell Mass-Derived Lineages Depend on Sall4 Function

Eisenberger, Tobias, Treier, Mathias, Elling, Ulrich, Klasen, Christian, Anlag, Katrin

2006

Overview

Sa1l4 is a mammalian Spalt transcription factor expressed by cells of the early embryo and germ cells, an expression pattern similar to that of both Oct4 and Sox2, which play essential roles during early murine development. We show that the activity of Sa114 is cell-autonomously required for the development of the epiblast and primitive endoderm from the inner cell mass. Furthermore, no embryonic or extraembryonic endoderm stem cell lines could be established from Sall4-deficient blastocysts. In contrast, neither the development of the trophoblast lineage nor the ability to generate trophoblast cell lines from murine blastocysts was impaired in the absence of Sa114. These data establish Sa1l4 as an essential transcription factor required for the early development of inner cell mass-derived cell lineages.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Animals / Biological Sciences / Blastocyst / Blastocyst Inner Cell Mass - cytology / Blastocyst Inner Cell Mass - metabolism / Cell Lineage / Cell lines / Cells / Cells, Cultured / Cellular immunity / Cultured cells / DNA-Binding Proteins - genetics / DNA-Binding Proteins - metabolism / Embryonic cells / Embryos / Endoderm / Gene expression / Gene Expression Regulation, Developmental / Germ cells / Giant cells / In Vitro Techniques / Mice / Mutation - genetics / Stem cells / Transcription Factors - genetics / Transcription Factors - metabolism