Journal Article

Bisphenol S Impaired Human Granulosa Cell Steroidogenesis In Vitro

Innovations Thérapeutiques et Résistances (InTheRes) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT), Projet Région Centre Val de Loire BEMOL APR IR 2017Projet Agence de la BioMédecine 2018 FertiPhénol, ANR-18-CE34-0011,MAMBO,Impact du métabolisme sur les effets ovariens du bisphenol S, Elis, Sébastien, Guerif, Fabrice, Desmarchais, Alice, Maillard, Virginie, Amar, Sarah, Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Binet, Aurélien, Papillier, Pascal, Teteau, Ophélie, Lac

2020

Overview

Bisphenol S (BPS) is a structural analog of the endocrine disruptor bisphenol A (BPA); it is the main BPA replacement in the plastics industry. Previous studies have shown that BPA and BPS exhibit similar effects on reproduction in fish and rodent species. BPS reportedly alters steroidogenesis in bovine granulosa cells. Luteinised granulosa cells collected from 59 women who were undergoing an in vitro fertilization procedure were cultured for 48 h in the presence or absence of BPS (10 nM, 100 nM, 1 µM, 10 µM or 50 µM). BPS exposure was investigated by assessing follicular fluids from these 59 women for their BPS content. Culture medium, cells, total messenger RNA (mRNA) and total protein extracted from the luteinised granulosa cells were examined for oestradiol and progesterone secretion, cellular proliferation, viability, gene expression, steroidogenic enzyme expression and cell signaling. BPS was measured in follicular fluids using mass spectrometry. Exposure of granulosa cells to 10 or 50 µM BPS for 48 h induced a 16% (p = 0.0059) and 64% (p < 0.0001) decrease, respectively, in progesterone secretion; 50 µM BPS decreased oestradiol secretion by 46% (p < 0.0001). Ten µM BPS also tended to reduce CYP11A1 protein expression by 37% (p = 0.0947) without affecting HSD3B1 and CYP19A1 expression. Fifty µM BPS increased ERRγ expression. Environmental levels of BPS (nanomolar range) did not induce changes in steroidogenesis in human granulosa cells. The effects of BPS were observed after only 48 h of BPS exposure. These acute effects might be similar to chronic effects of physiological BPS levels.

• Publisher: MDPI,CCSD,MDPI AG
• Subject: Bisphenol A / Cell growth / Cells, Cultured / Cholesterol Side-Chain Cleavage Enzyme - genetics / Cholesterol Side-Chain Cleavage Enzyme - metabolism / Endocrine disruptors / Female / Fluids / Follicular Fluid - drug effects / Follicular Fluid - metabolism / Gene expression / Gene Expression Regulation - drug effects / Granulosa Cells - drug effects / Granulosa Cells - metabolism / Granulosa Cells - pathology / Heat resistance / Human health and pathology / Humans / In Vitro Techniques / Kinases / Life Sciences / Phenols - pharmacology / Phosphorylation / Progesterone - biosynthesis / Protein expression / Studies / Sulfones - pharmacology / Urine / Womens health
• ISBN: 0005249085002