Asset Details
Do you wish to reserve the book?
Generation of a Close-to-Native In Vitro System to Study Lung Cells–Extracellular Matrix Crosstalk
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Generation of a Close-to-Native In Vitro System to Study Lung Cells–Extracellular Matrix Crosstalk
Do you wish to request the book?
Generation of a Close-to-Native In Vitro System to Study Lung Cells–Extracellular Matrix Crosstalk
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Generation of a Close-to-Native In Vitro System to Study Lung Cells–Extracellular Matrix Crosstalk
2018
Overview
Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior.
In vitro
culture systems are often poor in ECM constituents, thus not allowing for naturally occurring cell–ECM interactions. This study reports on a straightforward and efficient method for the generation of ECM scaffolds from lung tissue and its subsequent
in vitro
application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions, and DNase. Resultant ECM scaffolds were devoid of cells and DNA, whereas lung ECM architecture of alveolar region and blood and airway networks were preserved. Scaffolds were predominantly composed of core ECM and ECM-associated proteins such as collagens I-IV, nephronectin, heparan sulfate proteoglycan core protein, and lysyl oxidase homolog 1, among others. When homogenized and applied as coating substrate, ECM supported the attachment of lung fibroblasts (LFs) in a dose-dependent manner. After ECM characterization and biocompatibility tests, a novel
in vitro
platform for three-dimensional (3D) matrix repopulation that permits live imaging of cell–ECM interactions was established. Using this system, LFs colonized the ECM scaffolds, displaying a close-to-native morphology in intimate interaction with the ECM fibers, and showed nuclear translocation of the mechanosensor yes-associated protein (YAP), when compared with cells cultured in two dimensions. In conclusion, we developed a 3D-like culture system, by combining an efficient decellularization method with a live-imaging culture platform, to replicate
in vitro
native lung cell–ECM crosstalk. This is a valuable system that can be easily applied to other organs for ECM-related drug screening, disease modeling, and basic mechanistic studies.
