Journal Article

Phenotypic Investigation of Virulence Factors, Susceptibility to Ceragenins, and the Impact of Biofilm Formation on Drug Efficacy in Candida auris Isolates from Türkiye

Alkan, Busra, Komec, Selda, Guzel, Cagla Bozkurt, Turan, Deniz, Genc, Gonca Erkose, Demir, Elif Sena, Aygun, Gokhan, Teke, Leyla, Erturan, Zayre, Savage, Paul B., Oyardi, Ozlem

2023

Overview

Candida auris has emerged as a significant fungal threat due to its rapid worldwide spread since its first appearance, along with its potential for antimicrobial resistance and virulence properties. This study was designed to examine virulence characteristics, the efficacy of ceragenins, and biofilm-derived drug resistance in seven C. auris strains isolated from Turkish intensive care patients. It was observed that none of the tested strains exhibited proteinase or hemolysis activity; however, they demonstrated weak phospholipase and esterase activity. In addition, all strains were identified as having moderate to strong biofilm formation characteristics. Upon determining the minimum inhibitory concentrations (MIC) of ceragenins, it was discovered that CSA-138 exhibited the highest effectiveness with a MIC range of 1–0.5 µg/mL, followed by CSA-131 with a MIC of 1 µg/mL. Also, antimicrobial agents destroyed mature biofilms at high concentrations (40–1280 µg/mL). The investigation revealed that the strains isolated from Türkiye displayed weak exoenzyme activities. Notably, the ceragenins exhibited effectiveness against these strains, suggesting their potential as a viable treatment option.

• Publisher: MDPI AG,MDPI
• Subject: Antimicrobial agents / Antimicrobial resistance / Biofilms / Candida auris / ceragenins / Drug resistance / Epidemics / Gram-positive bacteria / Hospitals / Infections / Laboratories / Minimum inhibitory concentration / Mortality / Pathogenesis / Pathogens / Virulence / Virulence factors