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Novel and effective screening system for recombinant protein production in CHO cells
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Novel and effective screening system for recombinant protein production in CHO cells
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Journal Article
Novel and effective screening system for recombinant protein production in CHO cells
2024
Overview
At present, biopharmaceuticals have received extensive attention from the society, among which recombinant proteins have a good growth trend and a large market share. Chinese hamster ovary (CHO) cells are the preferred mammalian system to produce glycosylated recombinant protein drugs. A highly efficient and stable cell screening method needs to be developed to obtain more and useful recombinant proteins. Limited dilution method, cell sorting, and semi-solid medium screening are currently the commonly used cell cloning methods. These methods are time-consuming and labor-intensive, and they have the disadvantage of low clone survival rate. Here, a method based on semi-solid medium was developed to screen out high-yielding and stable cell line within 3 weeks to improve the screening efficiency. The semi-solid medium was combined with an expression vector containing red fluorescent protein (RFP) for early cell line development. In accordance with the fluorescence intensity of RFP, the expression of upstream target gene could be indicated, and the fluorescence intensity was in direct proportion to the expression of upstream target gene. In conclusion, semi-solid medium combined with bicistronic expression vector provides an efficient method for screening stable and highly expressed cell lines.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/337
/ 631/61
/ Animals
/ Chinese hamster ovary (CHO) cell
/ Cloning
/ Humanities and Social Sciences
/ Luminescent Proteins - genetics
/ Luminescent Proteins - metabolism
/ Proteins
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Science
/ Survival
