Journal Article

A novel role of IGFBP5 in the migration, invasion and spheroids formation induced by IGF-I and insulin in MCF-7 breast cancer cells

Salazar, Eduardo Perez, Cortes-Reynosa, Pedro, Rodríguez-Ochoa, Nínive, Torres-Alamilla, Pablo, Rodríguez-Rojas, Karem

2024

Overview

Purpose The insulin-like growth factor (IGF) system includes IGF-I, IGF-II insulin and their membrane receptors. IGF system also includes a family of proteins namely insulin-like growth factor-binding proteins (IGFBPs) composed for six major members (IGFBP-1 to IGFBP6), which capture, transport and prolonging half-life of IGFs. However, it has been described that IGFBPs can also have other functions. Methods IGFBP5 expression was inhibited by shRNAs, migration was analyzed by scratch-wound assays, invasion assays were performed by the Boyden chamber method, spheroids formation assays were performed on ultra-low attachment surfaces, expression and phosphorylation of proteins were analyzed by Western blot. Results IGFBP5 is a repressor of IGF-IR expression, but it is not a repressor of IR in MCF-7 breast cancer cells. In addition, IGFBP5 is a suppressor of migration and MMP-9 secretion induced by IGF-I and insulin, but it does not regulate invasion in MCF-7 cells. IGFBP5 also is a repressor of MCF-7 spheroids formation. However treatment with 340 nM rescues the inhibitory effect of IGFBP in the MCF-7 spheroids formation. Conclusion IGFBP5 regulates IGF-IR expression, migration and MMP-9 secretion induced by IGF-I and/or insulin, and the spheroids formation in MCF-7 breast cancer cells.

• Publisher: Springer US,Springer,Springer Nature B.V
• Subject: Analysis / Binding proteins / Boyden chamber / Breast cancer / Breast Neoplasms - genetics / Breast Neoplasms - metabolism / Breast Neoplasms - pathology / Cancer cells / Cell migration / Cell Movement / Cell surface receptors / Female / Gelatinase B / Gene Expression Regulation, Neoplastic - drug effects / Growth factors / Humans / Insulin / Insulin - metabolism / Insulin receptors / Insulin-Like Growth Factor Binding Protein 5 - genetics / Insulin-Like Growth Factor Binding Protein 5 - metabolism / Insulin-like growth factor I / Insulin-Like Growth Factor I - metabolism / Insulin-Like Growth Factor I - pharmacology / Insulin-like growth factor I receptors / Insulin-like growth factor II / Insulin-like growth factor-binding protein 1 / Insulin-like growth factor-binding protein 5 / Insulin-like growth factor-binding protein 6 / Insulin-like growth factor-binding proteins / Insulin-like growth factors / Matrix Metalloproteinase 9 - metabolism / MCF-7 Cells / Medicine / Medicine & Public Health / Neoplasm Invasiveness / Oncology / Phosphorylation / Protein binding / Proteins / Receptor, IGF Type 1 - metabolism / Secretion / Spheroids / Spheroids, Cellular