Journal Article

Immunolocalization of mannitol dehydrogenase in celery plants and cells

Conkling M.A, Williamson J.D, Pharr D.M, Zamski E, Yamamoto Y.T

1996

Overview

Immunolocalization of mannitol dehydrogenase (MTD) in celery (Apium graveolens L.) suspension cells and plants showed that MTD is a cytoplasmic enzyme. MTD was found in the meristems of celery root apices, in young expanding leaves, in the vascular cambium, and in the phloem, including sieve-element/companion cell complexes, parenchyma, and in the exuding phloem sap of cut petioles. Suspension cells that were grown in medium with mannitol as the sole carbon source showed a high anti-MTD cross-reaction in the cytoplasm, whereas cells that were grown in sucrose-containing medium showed little or no cross-reaction. Gel-blot analysis of proteins from vascular and nonvascular tissues of mature celery petioles showed a strong anti-MTD sera cross-reactive band, corresponding to the 40-kD molecular mass of MTD in vascular extracts, but no cross-reactive bands in nonvascular extracts. The distribution pattern of MTD within celery plants and in cell cultures that were grown on different carbon sources is consistent with the hypothesis that the Mtd gene may be regulated by sugar repression. Additionally, a developmental component may regulate the distribution of MTD within celery plants.

• Publisher: American Society of Plant Physiologists
• Subject: actividad enzimatica / activite enzymatique / alcohol dehydrogenase / alcohol deshidrogenasa / alcohol oxidoreductases / alcool deshydrogenase / analysis / Animals / Antibody Specificity / Apium graveolens / cambium / carbon / carbone / carbono / Celery / cell culture / cell suspension culture / Cells, Cultured / Cross reaction / Cross Reactions / cultivo de celulas / culture de cellule / cytology / Cytoplasm / Cytoplasm - enzymology / Cytoplasm - ultrastructure / enzyme activity / enzymic activity / enzymology / etapas de desarrollo de la planta / expresion genica / expression des genes / feuille / fisiologia vegetal / floema / gene expression / genetica / genetics / genetique / hojas / Immunoblotting / Immunohistochemistry / Leaves / manitol / mannitol / Mannitol Dehydrogenases / Mannitol Dehydrogenases - analysis / meristemas / meristeme / meristems / nutrientes / nutrients / Petioles / Phloem / Phloem companion cells / phloeme / physiologie vegetale / plant development / plant developmental stages / Plant Leaves / plant physiology / Plant Roots / plant vascular system / Plants / Rabbits / racine / raices / root meristems / Root tips / roots / Sieve elements / sistema vascular de la planta / stade de developpement vegetal / substance nutritive / Sugars / systeme vasculaire des plantes / translocation (plant physiology) / ultrastructure / Vegetables / Vegetables - cytology / Vegetables - enzymology / Whole Plant, Environmental, and Stress Physiology