Journal Article

Paralogous Genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 Have Partially Redundant Functions during Arabidopsis Development

Lamb, Rebecca S, Teotia, Sachin

2009

Overview

RADICAL-INDUCED CELL DEATH1 (RCD1) and SIMILAR TO RCD ONE1 (SRO1) are the only two proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome containing both a putative poly(ADP-ribose) polymerase catalytic domain and a WWE protein-protein interaction domain, although similar proteins have been found in other eukaryotes. Poly(ADP-ribose) polymerases mediate the attachment of ADP-ribose units from donor NAD⁺ molecules to target proteins and have been implicated in a number of processes, including DNA repair, apoptosis, transcription, and chromatin remodeling. We have isolated mutants in both RCD1 and SRO1, rcd1-3 and sro1-1, respectively. rcd1-3 plants display phenotypic defects as reported for previously isolated alleles, most notably reduced stature. In addition, rcd1-3 mutants display a number of additional developmental defects in root architecture and maintenance of reproductive development. While single mutant sro1-1 plants are relatively normal, loss of a single dose of SRO1 in the rcd1-3 background increases the severity of several developmental defects, implying that these genes do share some functions. However, rcd1-3 and sro1-1 mutants behave differently in several developmental events and abiotic stress responses, suggesting that they also have distinct functions. Remarkably, rcd1-3; sro1-1 double mutants display severe defects in embryogenesis and postembryonic development. This study shows that RCD1 and SRO1 are at least partially redundant and that they are essential genes for plant development.

• Publisher: American Society of Plant Biologists
• Subject: abiotic stress / active sites / Alleles / Amino Acid Sequence / apoptosis / Arabidopsis / Arabidopsis - genetics / Arabidopsis - growth & development / Arabidopsis - metabolism / Arabidopsis Proteins / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / Biological and medical sciences / Cloning, Molecular / Development and Hormone Action / DNA repair / Embryos / eukaryotic cells / Fundamental and applied biological sciences. Psychology / Gene Expression Profiling / Gene Expression Regulation, Developmental / Gene Expression Regulation, Developmental - physiology / Gene Expression Regulation, Plant / Gene Expression Regulation, Plant - physiology / Genes / genetics / Genotype / growth & development / Hypocotyls / Leaves / metabolism / Molecular Sequence Data / mutants / Mutation / Nuclear Proteins / Nuclear Proteins - genetics / Nuclear Proteins - metabolism / Phenotype / Phenotypes / physiology / plant development / Plant Epidermis / Plant Epidermis - ultrastructure / Plant physiology and development / Plant roots / Plants / Poly(ADP-ribose) Polymerases / Poly(ADP-ribose) Polymerases - genetics / Poly(ADP-ribose) Polymerases - metabolism / protein-protein interactions / proteins / Reproduction / Reproduction - physiology / roots / Seedlings / Seeds / Seeds - genetics / Seeds - metabolism / Seeds - ultrastructure / stress response / Stress, Physiological / Stress, Physiological - physiology / ultrastructure