Journal Article

Establishment of a resource population of SLA haplotype-defined Korean native pigs

Smith, Douglas M., University of Michigan, Ann Arbor, MI, USA, Lee, Y.J., Chungnam National University, Daejeon, Republic of Korea, Cho, H.O., Chungnam National University, Daejeon, Republic of Korea, Ho, C.S., University of Michigan, Ann Arbor, MI, USA, Jeon, J.T., Gyeongsang National University, Jinju, Republic of Korea, Park, C.K., Konkuk University, Seoul, Republic of Korea, Lee, J.H., Chungnam National University, Daejeon, Republic of Korea, Cho, I.C., Subtropical Animal Experiment Station, National Institute of Animal Science, RDA, Jeju, Republic of Korea, Ko, M.S., Subtropical Animal Experiment Station, National Institute of Animal Science, RDA, Jeju, Republic of Korea, Lee, S.S., Subtropical Animal Experiment Station, National Institute of Animal Science, RDA, Jeju, Republic of Korea

2010

Overview

The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens are also important targets for immunological recognition of foreign tissue grafts. We recently established a resource population of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 animals derived from three generations of the Korean native pigs were genotyped for three SLA class Ⅰ (SLA-2, SLA-3 and SLA-1) and three SLA class Ⅱ loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total of seven SLA haplotypes (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class Ⅰ and five unique class Ⅱ haplotypes, were characterized in the founding animals. Class Ⅰ haplotype Lr-65.0 and class Ⅱ haplotype Lr-0.34 were novel; and together with Lr-56.0 these haplotypes appeared to be breed-specific. In the progeny population, Lr-7.23 and Lr-56.30 appeared to be the most prevalent haplotypes with frequencies of 34.7% and 31.6%, respectively; the overall homozygosity was 27.4%. This resource population of SLA-defined Korean native pigs will be useful as large animal models for various transplantation and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease resistance and production traits.

• Publisher: Korean Society for Molecular and Cellular Biology,한국분자세포생물학회
• Subject: Biochemistry / Biomedical and Life Sciences / Biomedicine / Biotechnology / Cell Biology / CERDO / genotyping / Korean native pigs / Life Sciences / PCR-SSP / PORCIN / SLA haplotype / SWINE / swine leukocyte antigen / 생물학